Department of Chemical Engineering, Institute of Chemical Technology, Matunga (E), Mumbai 400019, India.
Department of Chemical Engineering, Institute of Chemical Technology, Matunga (E), Mumbai 400019, India.
Int J Biol Macromol. 2019 Oct 1;138:1035-1043. doi: 10.1016/j.ijbiomac.2019.07.099. Epub 2019 Jul 17.
Enzyme embedded metal-organic frameworks (MOFs) are blooming in the field of enzyme immobilization on account of their superior catalytic efficiency, thermal and chemical stability as compared to native enzyme. However, the separation and recovery of enzyme embedded MOF composite is quite challenging due to its nanometer size, high dispersity and low density which limits the reusability. In this work, α-amylase embedded zeolite imidazole framework (ZIF)-67 was fabricated onto melamine sponge using surfactant assisted dip coating technique. This was achieved in single pot making the method facile and rapid. α-Amylase MOF-sponge was analysed and characterized by FT-IR, XRD, SEM and TGA. We demonstrated that the encapsulation could maintain the active conformational structure of α-amylase after immobilization procedure with excellent bioactivity. Further, apart from merits of framework shielding, it offers mass transfer limitation for macromolecular substrate (like starch) which was determined and expressed as effectiveness factor (η) by using Michaelis-Menten enzyme kinetics. At the end, recycling studies were carried out for α-amylase MOF-sponge which showed 32% residual activity after six consecutive cycles. It is believed that this strategy has great potential in the field of continuous biotransformation (catalysis), biosensing and biomedicine application.
酶嵌入金属有机骨架（MOFs）在酶固定化领域中蓬勃发展，因为与天然酶相比，它们具有更高的催化效率、热稳定性和化学稳定性。然而，由于酶嵌入 MOF 复合材料的纳米尺寸、高分散性和低密度，其分离和回收具有挑战性，这限制了其可重复使用性。在这项工作中，使用表面活性剂辅助浸渍涂层技术将α-淀粉酶嵌入沸石咪唑骨架（ZIF）-67 制备到三聚氰胺海绵上。这是在一锅法中完成的，使方法简单快速。通过 FT-IR、XRD、SEM 和 TGA 对α-淀粉酶 MOF-海绵进行了分析和表征。我们证明，封装可以在固定化过程后保持α-淀粉酶的活性构象结构，具有优异的生物活性。此外，除了框架屏蔽的优点外，它还为大分子底物（如淀粉）提供了质量传递限制，这可以通过使用米氏酶动力学来确定并表示为有效因子（η）。最后，对α-淀粉酶 MOF-海绵进行了回收研究，结果表明在连续六次循环后仍保留 32%的残余活性。人们相信，这种策略在连续生物转化（催化）、生物传感和生物医学应用领域具有巨大的潜力。
