Braude I A, Hochberg M C, Arnett F C, Waldmann T A
Meloy Laboratories, Springfield, VA.
J Rheumatol. 1988 Mar;15(3):438-44.
The immunoregulator human gamma interferon (IFN-gamma) suppressed the spontaneous in vitro synthesis and secretion of anti-DNA antibodies by peripheral blood mononuclear cells of patients with systemic lupus erythematosus (SLE-PBMC). Comparable level of suppression were observed with both natural human IFN-gamma and recombinant derived human IFN-gamma. In addition, the inhibitory effects of human IFN-gamma were completely neutralized by a monoclonal antibody directed against it. Human IFN-gamma also inhibited the antigen induced production of anti-DNA synthesis by SLE-PBMC. Based on the kinetics of inhibition, human IFN-gamma appeared to be acting directly on the B cell. Lastly, human IFN-gamma also suppressed the spontaneous production of IgG and IgM by SLE-PBMC. Our findings support the conclusion that SLE Ig production can be regulated and that human IFN-gamma may be clinically useful in the treatment of SLE as well as other immune complex diseases.
免疫调节剂人γ干扰素（IFN-γ）抑制了系统性红斑狼疮患者外周血单个核细胞（SLE-PBMC）体外自发合成和分泌抗DNA抗体。天然人IFN-γ和重组人IFN-γ均观察到了相当程度的抑制作用。此外，人IFN-γ的抑制作用被一种针对它的单克隆抗体完全中和。人IFN-γ还抑制了抗原诱导的SLE-PBMC抗DNA合成。根据抑制动力学，人IFN-γ似乎直接作用于B细胞。最后，人IFN-γ也抑制了SLE-PBMC自发产生IgG和IgM。我们的研究结果支持以下结论：SLE免疫球蛋白的产生可以被调节，并且人IFN-γ在SLE以及其他免疫复合物疾病的治疗中可能具有临床应用价值。
