CAS Key Laboratory of Urban Pollutant Conversion, Department of Applied Chemistry, University of Science & Technology of China, Hefei, 230026, China.
CAS Key Laboratory of Urban Pollutant Conversion, Department of Applied Chemistry, University of Science & Technology of China, Hefei, 230026, China; Anhui Province Key Laboratory of Polar Environment and Global Change, University of Science & Technology of China, Hefei, 230026, China.
J Hazard Mater. 2019 Dec 15;380:120873. doi: 10.1016/j.jhazmat.2019.120873. Epub 2019 Jul 12.
A Gram-negative chlorate-reducing bacterial strain XM-1 was isolated. The 16S rRNA gene sequence identified the isolate as Ochrobactrum anthropi XM-1, which was the first strain of genus Ochrobactrum reported having the ability to reduce chlorate. The optimum growth temperature and pH for strain XM-1 to reduce chlorate was found to be 30 °C and 5.0-7.5, respectively, under anaerobic condition. Strain XM-1 could tolerate high chlorate concentration (200 mM), and utilize a variety of carbohydrates (glucose, L-arabinose, D-fructose, sucrose), glycerin and sodium citrate as electron donors. In addition, oxygen and nitrate could be used as electron acceptors, but perchlorate could not be reduced. Enzyme activities related to chlorate reducing were characterized in cell extracts. Activities of chlorate reductase and chlorite dismutase could be detected in XM-1 cells grown under both aerobic and anaerobic conditions, implying the two enzymes were constitutively expressed. This work suggests a high potential of applying Ochrobactrum anthropi XM-1 for remediation of chlorate contamination.
一株能还原氯酸盐的革兰氏阴性菌 XM-1 被分离出来。通过 16S rRNA 基因序列鉴定，该菌株为人类食酸菌 XM-1，这是首例报道具有还原氯酸盐能力的食酸菌属菌株。在厌氧条件下，菌株 XM-1 还原氯酸盐的最适生长温度和 pH 值分别为 30°C 和 5.0-7.5。XM-1 菌株能耐受高浓度的氯酸盐（200mM），并能利用多种碳水化合物（葡萄糖、L-阿拉伯糖、D-果糖、蔗糖）、甘油和柠檬酸钠作为电子供体。此外，氧气和硝酸盐可作为电子受体，但不能还原高氯酸盐。在细胞提取物中对与氯酸盐还原相关的酶活性进行了表征。在有氧和厌氧条件下培养的 XM-1 细胞中均能检测到氯酸盐还原酶和亚氯酸盐歧化酶的活性，表明这两种酶是组成型表达的。这项工作表明，人类食酸菌 XM-1 具有很高的应用潜力，可用于修复氯酸盐污染。
