González R G, Miglior S, Von Saltza I, Buckley L, Neuringer L J, Cheng H M
Francis Bitter National Magnet Laboratory, Massachusetts Institute of Technology, Cambridge 02139.
Magn Reson Med. 1988 Apr;6(4):435-44. doi: 10.1002/mrm.1910060409.
Phosphorus-31 (31P) nuclear magnetic resonance (NMR) spectroscopic analyses of the crystalline lens from the experimental diabetic rat were performed. Qualitative and quantitative alterations in the phosphorus-31 NMR metabolic profile were observed over the course of 3 weeks after the induction of diabetes mellitus. Most striking was the appearance of two new, as yet unidentified, metabolites. These metabolites which resonate at 6.6 and 5.8 ppm were not detected in the normal lens. Compared to the normal lens, glycerol-3-phosphate (G3P) underwent an eightfold increase in concentration and phosphorylcholine decreased to one-third its initial level. The phosphodiesters, glycerophosphorylcholine (GPC) and glycerophosphorylethanolamine (GPE), decreased to barely detectable levels. Oral treatment of the diabetic animal with an aldose reductase inhibitor resulted in the preservation of an essentially normal lens 31P NMR spectrum. Except for the changes observed in glycerol-3-phosphate, these alterations have not been previously reported and raise new questions about the metabolic consequences of diabetes mellitus and the dependence of these alterations on the action of a single enzyme, aldose reductase.
对实验性糖尿病大鼠的晶状体进行了磷-31（31P）核磁共振（NMR）光谱分析。在诱导糖尿病后的3周内，观察到了磷-31 NMR代谢谱的定性和定量变化。最显著的是出现了两种新的、尚未鉴定的代谢物。这些在6.6和5.8 ppm处共振的代谢物在正常晶状体中未被检测到。与正常晶状体相比，磷酸甘油（G3P）浓度增加了八倍，磷酸胆碱降至其初始水平的三分之一。磷酸二酯，甘油磷酸胆碱（GPC）和甘油磷酸乙醇胺（GPE），降至几乎无法检测的水平。用醛糖还原酶抑制剂对糖尿病动物进行口服治疗，可使晶状体31P NMR光谱基本保持正常。除了在磷酸甘油中观察到的变化外，这些改变以前尚未见报道，这对糖尿病的代谢后果以及这些改变对单一酶醛糖还原酶作用的依赖性提出了新的问题。
