MRI 报告基因 MagA 抑制转铁蛋白受体并定位 Fe 依赖性肺癌。

MRI reporter gene MagA suppresses transferrin receptor and maps Fe dependent lung cancer.

机构信息

Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomat ology Hospital, Department of Biomedical Engineering, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, China.

State Key Laboratory of Respiratory Disease, The Sixth Affiliated Hospital, Department of Biomedical Engineering, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, China.

出版信息

Nanomedicine. 2019 Oct;21:102064. doi: 10.1016/j.nano.2019.102064. Epub 2019 Jul 18.

DOI:10.1016/j.nano.2019.102064

PMID:31326524

Abstract

As a magnetic resonance imaging (MRI) reporter gene, MagA has become a powerful tool to monitor dynamic gene expression and allowed concomitant high resolution anatomical and functional imaging of subcellular genetic information. Here we establish a stably expressed MagA method for lung cancer MRI. The results show that MagA can not only enhance both in vitro and in vivo MRI contrast by specifically alternating the transverse relaxation rate of water, but also inhibit the malignant growth of lung tumor. In addition, MagA can regulate magnetic nanoparticle production in grafted tissues and also suppress transferrin receptor expression by acting as an iron transporter, and meanwhile can permit iron biomineralization in the presence of mammalian iron homeostasis. This work provides experimental evidence for the safe preclinical applications of MagA as both a potential inhibitor and an MRI-based tracing tool for iron ion-dependent lung cancer.

摘要

作为一种磁共振成像（MRI）报告基因，MagA 已成为监测动态基因表达的有力工具，并允许对亚细胞遗传信息进行同时的高分辨率解剖学和功能成像。在这里，我们建立了一种稳定表达 MagA 的方法用于肺癌 MRI。结果表明，MagA 不仅可以通过特异性改变水的横向弛豫率来增强体外和体内 MRI 对比，还可以抑制肺癌肿瘤的恶性生长。此外，MagA 可以调节移植组织中的磁性纳米颗粒的产生，并通过作为铁转运体来抑制转铁蛋白受体的表达，同时可以在哺乳动物铁稳态存在的情况下允许铁的生物矿化。这项工作为 MagA 作为一种潜在的抑制剂和基于 MRI 的铁离子依赖性肺癌追踪工具的安全临床前应用提供了实验证据。