Kim Joo Yun, Jang Sung Sik, Lee Jung Lyoul, Sim Jae-Hun, Shim Jae-Jung
R&BD Center, Korea Yakult Co. Ltd., Gyeonggi 17086, Korea.
College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea.
Prev Nutr Food Sci. 2019 Jun;24(2):159-164. doi: 10.3746/pnf.2019.24.2.159. Epub 2019 Jun 30.
has been used in East Asia as a folk medicine for symptoms such as inflammation, allergy, and gastritis. Administration of extract to pylori-ligated rat stomachs reduces gastric acid secretion and alleviates esophagus damage caused by gastric reflux. Therefore, in this study we aimed to investigate whether extracts inhibit reflux esophagitis by blocking H histamine receptor (HR). Dimaprit, a HR specific agonist, induced intracellular cyclic adenosine monophosphate (cAMP) production in U937 cells. Pretreatment with extracts significantly blocked dimaprit-induced cAMP production in a concentration-dependent manner. To extracted with different ethanol concentrations to determine the optimum method. We found that the 70% ethanol extract showed the most potent HR antagonistic effect against dimaprit-induced cAMP production. However, water extract did not show any HR blocking effect. These findings suggest that extracted using ethanol specifically inhibits gastric acid secretion and reduces esophageal injury by blocking HR in a competitive manner. Therefore, extracts may be used in food or medicine to prevent HR-related diseases, such as gastric hyperacidity and reflux esophagitis.
在东亚地区，它作为一种民间药物，用于治疗炎症、过敏和胃炎等症状。将提取物施用于幽门结扎的大鼠胃部，可减少胃酸分泌，并减轻胃反流引起的食管损伤。因此，在本研究中，我们旨在探讨提取物是否通过阻断H组胺受体（HR）来抑制反流性食管炎。HR特异性激动剂地马普利可诱导U937细胞内产生细胞内环磷酸腺苷（cAMP）。提取物预处理以浓度依赖的方式显著阻断了地马普利诱导的cAMP产生。用不同乙醇浓度提取以确定最佳方法。我们发现70%乙醇提取物对地马普利诱导的cAMP产生表现出最有效的HR拮抗作用。然而，水提取物未显示出任何HR阻断作用。这些发现表明，用乙醇提取的提取物通过竞争性阻断HR来特异性抑制胃酸分泌并减少食管损伤。因此，提取物可用于食品或药物中，以预防与HR相关的疾病，如胃酸过多和反流性食管炎。
