State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
Chem Commun (Camb). 2019 Aug 28;55(67):9919-9922. doi: 10.1039/c9cc04533c. Epub 2019 Jul 22.
Reported herein is a relebactam-derived fluorogenic reagent for covalent labeling of serine β-lactamases (SBLs), which are the major causes of bacterial resistance to β-lactam antibiotics. This highly selective imaging reagent generates over 300-fold stronger near-infrared fluorescence signals upon covalently bonding to SBLs, allowing wash-free visualization of live antimicrobial-resistant bacteria.
本文报道了一种雷巴他定衍生的荧光探针，用于共价标记丝氨酸β-内酰胺酶（SBLs），SBLs 是导致细菌对β-内酰胺类抗生素产生耐药性的主要原因。这种高度选择性的成像试剂与 SBLs 发生共价键合后会产生超过 300 倍的近红外荧光信号，可实现无需洗涤即可实时可视化抗微生物耐药菌。
