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基于有机分子的免洗荧光工具：设计原理与生物医学应用

Wash-free fluorescent tools based on organic molecules: Design principles and biomedical applications.

作者信息

Tan Jingyun, Wang Chunfei, Hu Zhangjun, Zhang Xuanjun

机构信息

Faculty of Health Sciences University of Macau Macau China.

Department of Pharmacology School of Pharmacy Wannan Medical College Wuhu China.

出版信息

Exploration (Beijing). 2024 Jun 28;5(1):20230094. doi: 10.1002/EXP.20230094. eCollection 2025 Feb.

DOI:10.1002/EXP.20230094
PMID:40040824
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11875451/
Abstract

Fluorescence-assisted tools based on organic molecules have been extensively applied to interrogate complex biological processes in a non-invasive manner with good sensitivity, high resolution, and rich contrast. However, the signal-to-noise ratio is an essential factor to be reckoned with during collecting images for high fidelity. In view of this, the wash-free strategy is proven as a promising and important approach to improve the signal-to-noise ratio, thus a thorough introduction is presented in the current review about wash-free fluorescent tools based on organic molecules. Firstly, generalization and summarization of the principles for designing wash-free molecular fluorescent tools (WFTs) are made. Subsequently, to make the thought of molecule design more legible, a wash-free strategy is highlighted in recent studies from four diverse but tightly binding aspects: (1) special chemical structures, (2) molecular interactions, (3) bio-orthogonal reactions, (4) abiotic reactions. Meanwhile, biomedical applications including bioimaging, biodetection, and therapy, are ready to be accompanied by. Finally, the prospects for WFTs are elaborated and discussed. This review is a timely conclusion about wash-free strategy in the fluorescence-guided biomedical applications, which may bring WFTs to the forefront and accelerate their extensive applications in biology and medicine.

摘要

基于有机分子的荧光辅助工具已被广泛应用于以非侵入性方式探究复杂的生物过程，具有良好的灵敏度、高分辨率和丰富的对比度。然而，在采集高保真图像时，信噪比是一个必须考虑的重要因素。鉴于此，免洗策略被证明是提高信噪比的一种有前景且重要的方法，因此在本综述中对基于有机分子的免洗荧光工具进行了全面介绍。首先，对免洗分子荧光工具（WFTs）的设计原理进行了概括和总结。随后，为了使分子设计思路更加清晰，从四个不同但紧密相关的方面对近期研究中的免洗策略进行了重点介绍：（1）特殊化学结构，（2）分子相互作用，（3）生物正交反应，（4）非生物反应。同时，还将介绍包括生物成像、生物检测和治疗在内的生物医学应用。最后，对WFTs的前景进行了阐述和讨论。本综述是对荧光引导生物医学应用中免洗策略的及时总结，这可能会使WFTs处于前沿地位，并加速其在生物学和医学中的广泛应用。

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