Laboratory of Enzyme Engineering and Microbiology, National Engineering School of Sfax, University of Sfax, Sfax, Tunisia.
Laboratory of Microorganisms and Biomolecules, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia.
Environ Toxicol. 2019 Nov;34(11):1165-1176. doi: 10.1002/tox.22818. Epub 2019 Jul 21.
The present study aimed to evaluate the potential protective and antioxidant effect of polysaccharides (PS) extracted from Ulva lactuca against thiacloprid (THC) induced nephrotoxicity and hepatotoxicity. The antioxidant capacity of PS was tested in vitro using ABTS radical scavenging activity and plasmid DNA cleavage assays andin vivo on adult male rats treated for 30 days. Animals were allocated into four groups: control; THC (22.5 mg/kg); THC (22.5 mg/kg) + PS1 (100 mg/kg diet); and THC (22.5 mg/kg) + PS2 (200 mg/kg diet). The structural features of PS were determined by Fourier transformed infrared (FT-IR), UV absorption peak detection, high performance liquid chromatography (HPLC) and gel permeation chromatography, and also functional properties were investigated. Overall, results indicated that THC increased significantly malondialdehyde, advanced oxidation protein products, glutathione levels, which is correlated with severe histological and plasmatic biochemical injuries in both liver and kidney tissues. However, cotreatment PS induced a significant protective and healing affects against the nephrotoxicity and hepatotoxcity induced by THC.
本研究旨在评估浒苔多糖（PS）对噻虫啉（THC）诱导的肾毒性和肝毒性的潜在保护和抗氧化作用。通过 ABTS 自由基清除活性和质粒 DNA 切割试验，在体外测试 PS 的抗氧化能力，并在成年雄性大鼠中进行 30 天的体内试验。动物被分为四组：对照组；THC（22.5mg/kg）；THC（22.5mg/kg）+PS1（100mg/kg 饮食）；和 THC（22.5mg/kg）+PS2（200mg/kg 饮食）。通过傅里叶变换红外（FT-IR）、紫外吸收峰检测、高效液相色谱（HPLC）和凝胶渗透色谱确定 PS 的结构特征，并研究其功能特性。总的来说，结果表明，THC 显著增加了丙二醛、高级氧化蛋白产物、谷胱甘肽水平，这与肝和肾组织的严重组织学和血浆生化损伤相关。然而，PS 的共同处理对 THC 诱导的肾毒性和肝毒性具有显著的保护和修复作用。
