Bartha K, Wojta J, Wagner O F, Binder B R
Department of Medical Physiology, University of Vienna, Austria.
Am J Physiol. 1988 Jun;254(6 Pt 2):R885-90. doi: 10.1152/ajpregu.1988.254.6.R885.
Human aortic (HAE), human umbilical vein (HUVE), and bovine aortic (BAE) endothelial cells were compared in their synthesis and release of fibrinolytic components during culturing. After isolation, the cultures were grown to confluency and then studied under identical conditions for release of tissue plasminogen activator (t-PA) antigen and plasminogen activator inhibitor (PAI) into serum-free medium. HAE cells released 10 times more t-PA antigen than HUVE cells, and the respective cell lysates also contained comparably higher values. Free PAI capacity was found in the conditioned media of both HAE and HUVE cells. BAE cell t-PA release was much lower than that of the HAE cells, and free inhibitor capacity was not found in the conditioned medium. BAE cells contained significant amounts of PA activity in cell membrane-bound form. This PA activity on the cell surface was not stimulated by addition of CNBr fibrinogen fragments but could be partially inhibited by activated bovine PAI and antibodies against human t-PA and urokinase PA, respectively.
对人主动脉内皮细胞（HAE）、人脐静脉内皮细胞（HUVE）和牛主动脉内皮细胞（BAE）在培养过程中纤维蛋白溶解成分的合成和释放情况进行了比较。分离后，将培养物培养至汇合状态，然后在相同条件下研究组织纤溶酶原激活物（t-PA）抗原和纤溶酶原激活物抑制剂（PAI）向无血清培养基中的释放情况。HAE细胞释放的t-PA抗原比HUVE细胞多10倍，相应的细胞裂解物中也含有相对较高的值。在HAE和HUVE细胞的条件培养基中均发现了游离PAI活性。BAE细胞的t-PA释放量远低于HAE细胞，且在条件培养基中未发现游离抑制剂活性。BAE细胞含有大量以细胞膜结合形式存在的PA活性。细胞表面的这种PA活性不受CNBr纤维蛋白原片段添加的刺激，但分别可被活化的牛PAI以及抗人t-PA和尿激酶型PA的抗体部分抑制。
