钙离子载体和佛波醇肉豆蔻酸酯协同通过不依赖前列腺素的机制抑制关节软骨细胞中蛋白聚糖的生物合成。
Calcium ionophore and phorbol myristate acetate synergistically inhibited proteoglycan biosynthesis in articular chondrocytes by prostaglandin independent mechanism.
作者信息
Bouakka M, Legendre P, Jouis V, Langris M, Béliard R, Loyau G, Bocquet J
机构信息
Laboratoire de Biochimie, UA CNRS 609, Caen, France.
出版信息
Biochem Biophys Res Commun. 1988 Jun 16;153(2):690-8. doi: 10.1016/s0006-291x(88)81150-1.
In rabbit articular chondrocytes, phorbol myristate acetate (PMA), 1,2-dioctanoyl-sn-glycerol (DG) and calcium ionophore (A23187), reduced the proteoglycan synthesis, in a dose-dependent manner. The combined treatment by PMA and A23187 resulted in an enhanced inhibition of proteoglycan production, indicating a synergistic effect. In presence of PMA or A23187, the release of prostaglandin E2 (PGE2) was dramatically increased. The addition of indomethacin and BW755c to chondrocytes stimulated by PMA or A23187, suppressed the liberation of PGE2, but did not stop the decrease of proteoglycan synthesis.
在兔关节软骨细胞中，佛波酯（PMA）、1,2 - 二辛酰 - sn - 甘油（DG）和钙离子载体（A23187）以剂量依赖的方式降低蛋白聚糖的合成。PMA和A23187联合处理导致对蛋白聚糖产生的抑制作用增强，表明存在协同效应。在PMA或A23187存在的情况下，前列腺素E2（PGE2）的释放显著增加。将吲哚美辛和BW755c添加到受PMA或A23187刺激的软骨细胞中，可抑制PGE2的释放，但不能阻止蛋白聚糖合成的减少。
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