School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, PR China.
Int J Syst Evol Microbiol. 2019 Oct;69(10):3087-3092. doi: 10.1099/ijsem.0.003593. Epub 2019 Jul 23.
Four strains, NYNU 15610, NYNU 15612, NYNU 15613 and NYNU 15615, of a novel ascomycetous yeast were isolated from the gut of (Coleoptera: Scarabeidae) collected from two different localities in Henan Province, Central PR China. The four strains shared identical sequences in both of the D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer regions. Sequence analyses revealed that this novel species represents a member of the genus . It differed from its closest known species , and , by 8.4-9.2 % sequence divergence (33-40 nt substitutions and 7-12 gaps over 509 bases) in the D1/D2 sequences. The formation of ascospores was not observed on various sporulation media. In contrast to , and , the novel yeast species was unable to assimilate succinate, ethanol, ethylamine, cadaverine and 10 % NaCl plus 5 % glucose, but was able to grow in vitamin-free medium. The name f.a., sp. nov. is proposed to accommodate these strains, with NYNU 15613 as the holotype.
从中国中部河南省两个不同地点采集的（鞘翅目：金龟子科）肠道中分离出四种新型子囊酵母菌株，分别为 NYNU 15610、NYNU 15612、NYNU 15613 和 NYNU 15615。这四种菌株在大亚基 rRNA 基因的 D1/D2 结构域和内部转录间隔区都具有相同的序列。序列分析表明，这种新型物种代表了属的一个成员。它与最接近的已知种和有 8.4-9.2%的序列差异（33-40 个核苷酸取代和 509 个碱基中的 7-12 个空位）。在各种产孢培养基上都没有观察到子囊孢子的形成。与和不同的是，新型酵母物种不能同化琥珀酸盐、乙醇、乙胺、尸胺和 10%NaCl+5%葡萄糖，但能够在无维生素培养基中生长。建议使用名称来容纳这些菌株，其中 NYNU 15613 为模式种。
