Wang Yun, Ren Yong-Cheng, Zhang Zheng-Tian, Ke Tao, Hui Feng-Li
School of Life Science and Technology, Nanyang Normal University,Nanyang 473061, PRChina.
Int J Syst Evol Microbiol. 2016 May;66(5):2008-2012. doi: 10.1099/ijsem.0.000979. Epub 2016 Feb 19.
During an investigation of yeasts associated with insects, three strains of a d-xylose-fermenting yeast species were isolated from the gut of the host beetles Allomyrina dichotoma (Coleoptera: Scarabeidae) collected on the Baotianman National Nature Reserve, Nanyan, Henan Province, China. These strains formed two elongated ascospores, which were tapered and curved at the ends in persistent asci. Sequence analyses of the D1/D2 domains of the large subunit (LSU) and small subunit (SSU) rRNA genes showed that these new strains represent a phylogenetically distinct species in the Spathaspora clade. This novel species differed from the closest species, Candida lyxosophila NRRL Y-17539T, by a 6.7 % sequence divergence (31 substitutions and 7 gaps) in the D1/D2 LSU rRNA gene and a 1.2 % divergence (17 substitutions, 4 gaps) in the SSU rRNA gene. The novel species can also be distinguished from C. lyxosophila NRRL Y-17539T in terms of the ability to assimilate myo-inositol and to grow in the presence of 0.1 % cycloheximide, as well as the inability to assimilate citrate. The name Spathaspora allomyrinae sp. nov. is proposed for this species. The type strain is NYNU 1495T ( = CICC 33057T = CBS 13924T). The MycoBank number is MB 815071.
在一项与昆虫相关的酵母调查中，从中国河南省南阳市宝天曼国家级自然保护区采集的寄主甲虫双叉犀金龟（鞘翅目：金龟科）肠道中分离出三株发酵D-木糖的酵母菌株。这些菌株形成两个细长的子囊孢子，在持久的子囊中，子囊孢子两端逐渐变细并弯曲。对大亚基（LSU）和小亚基（SSU）rRNA基因的D1/D2结构域进行序列分析表明，这些新菌株代表了Spathaspora分支中一个系统发育上独特的物种。这个新物种与最接近的物种嗜糖假丝酵母NRRL Y-17539T不同，在D1/D2 LSU rRNA基因中序列差异为6.7%（31个替换和7个缺口），在SSU rRNA基因中差异为1.2%（17个替换，4个缺口）。这个新物种在同化肌醇的能力、在0.1%放线菌酮存在下生长的能力以及不能同化柠檬酸盐方面也与嗜糖假丝酵母NRRL Y-17539T不同。为此物种提议命名为Spathaspora allomyrinae sp. nov.。模式菌株为NYNU 1495T（=CICC 33057T =CBS 13924T）。MycoBank编号为MB 815071。
