Academy of Agricultural Sciences, College of Plant Protection, Southwest University, Chongqing, China.
J Econ Entomol. 2019 Aug 3;112(4):1858-1865. doi: 10.1093/jee/toz087.
Tetranychus cinnabarinus (Boisduval), i.e., carmine spider mite, is a worldwide pest that can cause serious damage to plants. Problems of resistance have arisen since abamectin usage in the control of T. cinnabarinus. Unfortunately, there are only limited data on the extent of this problem. To understand the development of abamectin resistance in the carmine spider mite, we prokaryotically expressed an intradiol ring-cleavage dioxygenase (ID-RCD) gene sequence, TcID-RCD1, which had a significant upregulated expression of over 7.7 times in an abamectin-resistant strain (AbR) when compared with that of a susceptible strain (SS). The crude enzyme activity also indicated that the AbR had a higher activity than that exhibited in SS. When susceptible individuals were treated with abamectin, TcID-RCD1 was also overexpressed. Furthermore, using the RNA interference (RNAi) technique, TcID-RCD1 was successfully knocked down, with the expression level decreasing significantly to approximately 39% in the SS strain compared with the control. And the mortality of mites feeding on dsTcID-RCD1 increased significantly when treated with abamectin. These results strongly suggest that TcID-RCD1 is involved in abamectin resistance in T. cinnabarinus.
朱砂叶螨（Tetranychus cinnabarinus），又称红蜘蛛，是一种世界性的害虫，可对植物造成严重损害。自从阿维菌素被用于防治朱砂叶螨以来，已经出现了抗药性问题。不幸的是，关于这个问题的程度只有有限的数据。为了了解朱砂叶螨对阿维菌素抗药性的发展，我们原核表达了一个间二苯酚环裂解双加氧酶（ID-RCD）基因序列 TcID-RCD1，与敏感品系（SS）相比，在抗药性品系（AbR）中其表达水平显著上调了超过 7.7 倍。粗酶活性也表明 AbR 的活性高于 SS。当敏感个体用阿维菌素处理时，TcID-RCD1 也过表达。此外，利用 RNA 干扰（RNAi）技术，成功敲低了 TcID-RCD1，SS 品系的表达水平与对照相比显著下降约 39%。用阿维菌素处理喂食 dsTcID-RCD1 的螨虫死亡率显著增加。这些结果强烈表明 TcID-RCD1 参与了朱砂叶螨对阿维菌素的抗药性。
