Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), Academy of Agricultural Sciences, Southwest University, Chongqing, China.
Pest Manag Sci. 2024 Mar;80(3):1258-1265. doi: 10.1002/ps.7855. Epub 2023 Nov 20.
The citrus red mite, Panonychus citri (McGregor), a global pest of citrus, has developed different levels of resistance to various acaricides in the field. Abamectin is one of the most important insecticides/acaricides worldwide, targetting a wide number of insect and mite pests. The evolution of abamectin resistance in P. citri is threatening the sustainable use of abamectin for mite control.
The abamectin resistant strain (NN-Aba), derived from a field strain NN by consistent selection with abamectin, showed 4279-fold resistance to abamectin compared to a relatively susceptible strain (SS) of P. citri. Cross-resistance of NN-Aba was observed between abamectin and emamectin benzoate, pyridaben, fenpropathrin and cyflumetofen. Inheritance analyses indicated that abamectin resistance in the NN-Aba strain was autosomal, incompletely recessive and polygenic. The synergy experiment showed that abamectin toxicity was synergized by piperonyl butoxide (PBO), diethyl maleate (DEM) and tributyl phosphorotrithiotate (TPP) in the NN-Aba strain, and synergy ratios were 2.72-, 2.48- and 2.13-fold, respectively. The glutathione-S-transferases activity in the NN-Aba strain were significantly increased by 2.08-fold compared with the SS strain.
The abamectin resistance was autosomal, incompletely recessive and polygenic in P. citri. The NN-Aba strain showed cross-resistance to various acaricides with different modes of action. Metabolic detoxification mechanism participated in abamectin resistance in NN-Aba strain. These findings provide useful information for resistance management of P. citri in the field. © 2023 Society of Chemical Industry.
柑橘全爪螨（Panonychus citri ）是一种全球性的柑橘害虫，在田间已对各种杀螨剂产生了不同程度的抗性。阿维菌素是全球最重要的杀虫剂/杀螨剂之一，针对多种昆虫和螨虫害虫。柑橘全爪螨对阿维菌素的抗性进化正在威胁到阿维菌素防治螨虫的可持续使用。
从田间种群 NN 中通过持续阿维菌素选择获得的阿维菌素抗性种群（NN-Aba）与相对敏感种群（SS）相比，对阿维菌素的抗性高 4279 倍。NN-Aba 对阿维菌素和埃玛菌素苯甲酸、吡丙醚、甲氰菊酯和氟氯氰菊酯表现出交叉抗性。遗传分析表明，NN-Aba 种群的阿维菌素抗性是常染色体、不完全隐性和多基因的。增效实验表明，阿维菌素在 NN-Aba 种群中的毒性被增效剂胡椒基丁醚（PBO）、顺丁烯二酸二乙酯（DEM）和三丁基磷三硫代磷酸酯（TPP）增效，增效比分别为 2.72、2.48 和 2.13。与 SS 种群相比，NN-Aba 种群的谷胱甘肽-S-转移酶活性显著增加了 2.08 倍。
柑橘全爪螨的阿维菌素抗性是常染色体、不完全隐性和多基因的。NN-Aba 种群对具有不同作用模式的各种杀螨剂表现出交叉抗性。代谢解毒机制参与了 NN-Aba 种群对阿维菌素的抗性。这些发现为田间柑橘全爪螨的抗性管理提供了有用信息。© 2023 化学工业协会。
