Chebotarev A N, Golovina M E, Smirnova T D
Biull Eksp Biol Med. 1988 May;105(5):589-91.
The frequency of sister chromatid exchange and cell cycle duration were evaluated simultaneously. This approach is based on the analysis of distribution of cells with differential staining of sister chromatids after treatment of cells with 5-bromodeoxyuridine. The treatment of cells with thiotepa caused no changes in cell cycle duration, while the combination of thiotepa and hydroxyurea (HU) or cytosine-beta-D-arabinofuranoside (ARA-C) was observed to prolong cell cycle duration. Furthermore, it has been shown that caffeine, HU, ARA-C do not increase frequency of sister chromatid exchange in control cells and in cell treated with thiotepa.
同时评估了姐妹染色单体交换频率和细胞周期持续时间。该方法基于对用5-溴脱氧尿苷处理细胞后姐妹染色单体进行差异染色的细胞分布分析。用噻替派处理细胞不会导致细胞周期持续时间发生变化，而观察到噻替派与羟基脲（HU）或阿糖胞苷（ARA-C）联合使用会延长细胞周期持续时间。此外，研究表明，咖啡因、HU、ARA-C不会增加对照细胞和用噻替派处理的细胞中姐妹染色单体交换的频率。
