Department of Obstetrics and Gynecology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai 264000, Shandong, China.
Department of Obstetrics and Gynecology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai 264000, Shandong, China.
Biomed Pharmacother. 2019 Oct;118:109218. doi: 10.1016/j.biopha.2019.109218. Epub 2019 Jul 19.
Icariin is one of the most common active ingredients in traditional Chinese medicine, while its function against Premature ovarian failure (POF) has not been explored. POF animal model was induced by d-galactose, and icariin at different doses was administered. Ovarian structure and follicle counting were observed via hematoxylin and eosin staining. The levels of serum hormones were measured by ELISA. Primary ovarian granulosa cells were cultured to compare the protective effects of icariin on cell aging, and DNA damage markers including γH2AX and 53BP1 were assessed by Western Blot. Administration of icariin promoted ovary/body weight, follicles numbers and fertility outcomes. In addition, icariin downregulated the levels of follicle stimulating hormone and luteinizing hormone, and upregulated the levels of estradiol and anti-Müllerian hormone. Icariin protected ovarian granulosa cells from d-galactose induced aging, with increased cell viability and lower endogenous β-galactosidase activity. The alterations of expression level of γH2AX and 53BP1 by icariin indicated that the protection is via promoting DNA damage repair. In this study we tested the biological function of icariin against the d-galactose induced POF. Our results demonstrated that icariin effectively attenuated ovarian injury via promoting DNA damage repair, suggesting that icariin can be developed as a protective agent against POF.
淫羊藿苷是中药中最常见的活性成分之一，但其对卵巢早衰（POF）的作用尚未得到探索。通过 D-半乳糖诱导建立 POF 动物模型，并给予不同剂量的淫羊藿苷进行治疗。通过苏木精-伊红染色观察卵巢结构和卵泡计数。采用 ELISA 法检测血清激素水平。培养原代卵巢颗粒细胞，比较淫羊藿苷对细胞衰老的保护作用，并通过 Western blot 检测 DNA 损伤标志物γH2AX 和 53BP1 的表达。淫羊藿苷治疗可促进卵巢/体重比、卵泡数量和生育能力。此外，淫羊藿苷可下调卵泡刺激素和黄体生成素水平，上调雌二醇和抗苗勒管激素水平。淫羊藿苷可保护卵巢颗粒细胞免受 D-半乳糖诱导的衰老，增加细胞活力，降低内源性β-半乳糖苷酶活性。淫羊藿苷对γH2AX 和 53BP1 表达水平的改变表明，其通过促进 DNA 损伤修复发挥保护作用。本研究检测了淫羊藿苷对 D-半乳糖诱导的 POF 的生物学功能。结果表明，淫羊藿苷通过促进 DNA 损伤修复有效减轻卵巢损伤，提示淫羊藿苷可开发为防治 POF 的保护剂。
