Raja A, Baughman R P, Daniel T M
Department of Medicine, Case Western Reserve University, Cleveland.
Chest. 1988 Jul;94(1):133-7. doi: 10.1378/chest.94.1.133.
Bronchoalveolar lavage (BAL) samples from patients with pulmonary tuberculosis and from control subjects were studied using direct and inhibition enzyme-linked immunosorbent assay (ELISA) for the detection of IgG and IgA antibody to Mycobacterium tuberculosis culture filtrate and purified antigen 5 and for the detection of mycobacterial antigens. The IgG and IgA antibodies were found in the majority of samples from tuberculous patients. The IgG titers were higher than were IgA titers. Substantial nonspecificity was observed for antibodies to crude M tuberculosis culture filtrate. Antibodies to purified M tuberculosis antigen 5 were more specific. The detection of antibody in BAL did not offer advantages over previously described serodiagnostic assays. Antigen was found in some BAL samples from tuberculous patients. Greater specificity was observed with an assay based on purified antigen 5 than on crude mycobacterial culture filtrate.
采用直接和抑制酶联免疫吸附测定（ELISA），对肺结核患者和对照受试者的支气管肺泡灌洗（BAL）样本进行研究，以检测抗结核分枝杆菌培养滤液和纯化抗原5的IgG和IgA抗体以及检测分枝杆菌抗原。在大多数结核患者的样本中发现了IgG和IgA抗体。IgG滴度高于IgA滴度。观察到抗结核分枝杆菌粗培养滤液抗体存在大量非特异性。抗纯化结核分枝杆菌抗原5的抗体更具特异性。在BAL中检测抗体并不比先前描述的血清诊断试验更具优势。在一些结核患者的BAL样本中发现了抗原。基于纯化抗原5的检测方法比基于分枝杆菌粗培养滤液的检测方法具有更高的特异性。
