Balzer T, Sandforth F, Gutschmidt S, Riecken E O
Klinikum Steglitz, Department of Gastroenterology, Berlin, FRG.
Eur J Clin Invest. 1988 Apr;18(2):196-201. doi: 10.1111/j.1365-2362.1988.tb02413.x.
The purpose of this study was the investigation of changes in the lectin-binding pattern prior to tumour formation in an experimental model. Female Wistar rats were treated with 1,2-dimethylhydrazine (DMH). After 4, 8, 16, 24, 32 and 40 weeks of treatment the lectin-binding-pattern of the colonic mucosa appearing morphologically normal was examined at the caecum, proximal colon, distal colon and rectum, using FITC-conjugated Peanut-agglutinin (PNA) and Ulex europaeus-agglutinin1 (UEA1). In contradistinction to what has been reported earlier by other authors, PNA did not indicate constant cancer-associated mucin changes. In addition, there was no difference in the UEA1-binding between the control animals and the DMH-treated rats. Thus, in the rat there is no specific PNA- and UEA1-binding pattern during tumour induction in the gut.
本研究的目的是在一个实验模型中，调查肿瘤形成前凝集素结合模式的变化。用1,2 - 二甲基肼（DMH）处理雌性Wistar大鼠。在处理4、8、16、24、32和40周后，使用异硫氰酸荧光素（FITC）偶联的花生凝集素（PNA）和荆豆凝集素1（UEA1），检查盲肠、近端结肠、远端结肠和直肠处形态学上正常的结肠黏膜的凝集素结合模式。与其他作者先前报道的情况相反，PNA并未显示出与癌症相关的黏液持续变化。此外，对照动物和经DMH处理的大鼠之间UEA1结合没有差异。因此，在大鼠肠道肿瘤诱导过程中，不存在特定的PNA和UEA1结合模式。
