Department of Life Science, Dongguk University-Seoul, Goyang, 10326, South Korea.
Antonie Van Leeuwenhoek. 2019 Dec;112(12):1731-1738. doi: 10.1007/s10482-019-01302-7. Epub 2019 Jul 22.
A Gram-stain positive, short rod-shaped, aerobic, motile by means of gliding, yellow-pigmented actinobacterium, designated strain DD4a, was isolated from dry yellow foxtail. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DD4a is closely related to Amnibacterium soli MB78 (98.4% similarity), Amnibacterium kyonggiense KSL51201-037 (98.2%) and Amnibacterium endophyticum 1T4Z-3 (97.43%). Strain DD4a forms yellow colonies on R2A agar medium. The peptidoglycan was found to contains diaminopimelic acid (which is a diagnostic cell wall diamino acid), alanine, glutamic acid and lysine. The polar lipids diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids and an unidentified polar lipid were found to be present in strain DD4a. The major cellular fatty acids anteiso-C (42.9%) and iso-C (34.6%) were found in strain DD4a. The predominant respiratory quinones were found to be MK-11 and MK-12. The DNA G+C content of strain DD4a is 73.9 mol%. DNA-DNA relatedness of strain DD4a with A. soli MB78, A. kyonggiense KSL51201-037, and A. endophyticum 1T4Z-3 were 53.3% (± 1.1%), 47.0% (± 0.5%), and 47.9% (± 0.9%), respectively. The digital DNA-DNA hybridisation and average nucleotide identity values between strain DD4a and A. kyonggiense KSL51201-037 were determined to be 26.1% and 82.7%. On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, DD4a represents a novel member of the genus Amnibacterium, for which the name Amnibacterium setariae sp. nov., is proposed. The type strain of Amnibacterium setariae is DD4a (= KACC 19817 = JCM 32878).
一株革兰氏阳性、短杆状、需氧、能滑行运动的黄色素产生放线菌，被命名为 DD4a 菌株，从干燥的黄狗尾草中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，DD4a 菌株与 Solibacter soli MB78（相似度 98.4%）、Kongsongense KSL51201-037（相似度 98.2%）和内酰胺菌 1T4Z-3（相似度 97.43%）密切相关。DD4a 菌株在 R2A 琼脂培养基上形成黄色菌落。肽聚糖中含有二氨基庚二酸（这是一种诊断细胞壁二氨基庚二酸）、丙氨酸、谷氨酸和赖氨酸。在 DD4a 菌株中发现了二磷酸甘油酯、磷脂酰甘油、六种未鉴定的糖脂和一种未鉴定的极性脂。菌株 DD4a 中的主要细胞脂肪酸为anteiso-C（42.9%）和 iso-C（34.6%）。主要的呼吸醌为 MK-11 和 MK-12。菌株 DD4a 的 DNA G+C 含量为 73.9 mol%。DD4a 菌株与 Solibacter soli MB78、Kongsongense KSL51201-037 和内酰胺菌 1T4Z-3 的 DNA-DNA 同源性分别为 53.3%（±1.1%）、47.0%（±0.5%）和 47.9%（±0.9%）。通过数字 DNA-DNA 杂交和平均核苷酸同一性值的测定，DD4a 菌株与 Kongsongense KSL51201-037 的数值分别为 26.1%和 82.7%。基于表型、基因型、化学分类和系统发育分析，DD4a 代表 Amnibacterium 属的一个新成员，因此提议将其命名为 Amnibacterium setariae sp. nov.。Amnibacterium setariae 的模式菌株为 DD4a（=KACC 19817=JCM 32878）。
