Environmental Biotechnology Research Centre, Korea Research Institute of Bioscience & Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4750-4753. doi: 10.1099/ijs.0.052506-0. Epub 2013 Aug 29.
A Gram-stain-positive, aerobic, non-motile, non-spore-forming, short rod-shaped actinobacterium, designated strain PB243(T), was isolated from grass soil sampled in Daejeon, Republic of Korea. Comparative 16S rRNA gene sequence studies showed the isolate was clearly affiliated with the class Actinobacteria, and most closely related to Amnibacterium kyonggiense KEMC 51201-037(T), showing 98.8 % 16S rRNA gene sequence similarity. Cells of strain PB243(T) formed yellow colonies on R2A agar, contained MK-11 and MK-12 as the predominant menaquinones, l-2,4-diaminobutyric acid as the diagnostic cell-wall diamino acid, and anteiso-C15:0 and iso-C16:0 among the major fatty acids. The acyl type of the muramic acid was acetyl. The G+C content of the genomic DNA of strain PB243(T) was 71.5 mol%. Thus, the combined genotypic and phenotypic data supported the conclusion that strain PB243(T) represents a novel species of the genus Amnibacterium, for which the name Amnibacterium soli sp. nov. is proposed. The type strain is PB243(T) ( = KCTC 33147(T) = JCM 19015(T)).
一株革兰氏阳性、需氧、不运动、不产芽孢的短杆状放线菌，被命名为 PB243(T) 菌株，从韩国大田采集的草土中分离得到。比较 16S rRNA 基因序列研究表明，该分离物与放线菌纲明确相关，与 Amnibacterium kyonggiense KEMC 51201-037(T) 最为密切相关，其 16S rRNA 基因序列相似度为 98.8%。PB243(T) 菌株的细胞在 R2A 琼脂上形成黄色菌落，含有 MK-11 和 MK-12 作为主要的类异戊二烯醌，l-2,4-二氨基丁酸作为诊断细胞壁二氨基氨基酸，以及 anteiso-C15:0 和 iso-C16:0 作为主要脂肪酸。细胞壁 muramic 酸的酰基类型为乙酰基。PB243(T) 菌株的基因组 DNA 的 G+C 含量为 71.5 mol%。因此，综合表型和基因型数据支持 PB243(T) 菌株代表 Amnibacterium 属的一个新种的结论，建议将其命名为 Amnibacterium soli sp. nov.。该模式菌株为 PB243(T)（=KCTC 33147(T)=JCM 19015(T)）。
