Soliman Manar H, Ebaid Amany M
Department of Medical Microbiology & Immunology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Department of Rheumatology & Rehabilitation, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Egypt J Immunol. 2019 Jan;26(1):43-54.
Different cytokines play roles in the pathogenesis and tissue damage of Rheumatoid Arthritis (RA) including, Tumor necrosis factor superfamily (TNFSF) and their receptors particularly TNF-like ligand 1A (TL1A), and its decoy receptor DcR3. This study included 150 subjects, of them 50 patients having Rheumatoid Arthritis (RA), 50 patients with Osteoarthritis (OA), and 50 normal controls. Clinical examination was done and data was collected from patient's sheets, routine laboratory investigations included, rheumatoid factor (RF) antibody, anti-cyclic citrullinated peptide (anti-CCP) antibody, erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP). Disease activity score 28 was calculated and used to measure the activity of RA. Serum and synovial fluid (SF) TL1A and DcR3 levels were measured by (ELISA), while IL-17 was measured in supernatant fluid of PBMC culture after stimulation with recombinant human (rh) TL1A. Results showed significantly higher levels of TL1A and its decoy receptor DcR3 in RA patients than the other two groups. It was also found that TL1A is significantly related to the disease activity and enhances IL-17 production after stimulation of PBMC. These results can guide scientists to the future substitutions in the way of treatment of various inflammatory and autoimmune diseases.
不同的细胞因子在类风湿关节炎（RA）的发病机制和组织损伤中发挥作用，包括肿瘤坏死因子超家族（TNFSF）及其受体，特别是肿瘤坏死因子样配体1A（TL1A）及其诱饵受体DcR3。本研究纳入了150名受试者，其中50例类风湿关节炎（RA）患者、50例骨关节炎（OA）患者和50名正常对照。进行了临床检查，并从患者病历中收集数据，常规实验室检查包括类风湿因子（RF）抗体、抗环瓜氨酸肽（抗CCP）抗体、红细胞沉降率（ESR）和C反应蛋白（CRP）。计算疾病活动评分28并用于衡量RA的活动度。采用酶联免疫吸附测定法（ELISA）检测血清和滑液（SF）中TL1A和DcR3水平，而用重组人（rh）TL1A刺激后，在PBMC培养上清液中检测IL-17。结果显示，RA患者中TL1A及其诱饵受体DcR3水平显著高于其他两组。还发现TL1A与疾病活动度显著相关，并在刺激PBMC后增强IL-17的产生。这些结果可为科学家在未来治疗各种炎症性和自身免疫性疾病方面提供指导。
