癌胚抗原和非特异性交叉反应抗原基因在人结肠腺癌及正常结肠黏膜中的差异表达
Differential expression of carcinoembryonic antigen and nonspecific crossreacting antigen genes in human colon adenocarcinomas and normal colon mucosa.
作者信息
Sato C, Miyaki M, Oikawa S, Nakazato H, Kosaki G
机构信息
Department of Biochemistry, Tokyo Metropolitan Institute of Medical Science.
出版信息
Jpn J Cancer Res. 1988 Apr;79(4):433-7. doi: 10.1111/j.1349-7006.1988.tb01610.x.
Abstract
The level of mRNA for carcinoembryonic antigen (CEA) and nonspecific crossreacting antigen (NCA) in human colon adenocarcinomas and normal colon mucosa was analyzed by Northern blot hybridization using as probes 32P-labeled CEA cDNA and synthetic oligodeoxyribonucleotides specific to CEA and NCA mRNA sequences. The major 3.5-kb mRNA and a minor 4.2-kb mRNA are shown to be CEA-specific and expressed in both tissues, albeit at slightly different degrees, suggesting that the expression of CEA is regulated posttranscriptionally. Another minor mRNA of 2.9 kb is NCA-specific and expressed predominantly in cancerous tissues, suggesting its usefulness as a marker for colon cancer.
摘要
利用32P标记的癌胚抗原（CEA）cDNA以及针对CEA和非特异性交叉反应抗原（NCA）mRNA序列的合成寡脱氧核糖核苷酸作为探针，通过Northern印迹杂交分析了人结肠腺癌和正常结肠黏膜中CEA和NCA的mRNA水平。主要的3.5 kb mRNA和次要的4.2 kb mRNA被证明是CEA特异性的，且在两种组织中均有表达，尽管表达程度略有不同，这表明CEA的表达在转录后受到调控。另一种2.9 kb的次要mRNA是NCA特异性的，主要在癌组织中表达，这表明它可作为结肠癌的标志物。
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