用于测定葡萄干中黄曲霉毒素的四种薄层色谱法的比较。
Comparison of four thin-layer chromatographic methods for the determination of aflatoxins in raisins.
作者信息
Boyacioğlu D, Gönül M
机构信息
University of Ege, Engineering Faculty, Food Engineering Department, Izmir, Turkey.
出版信息
J Assoc Off Anal Chem. 1988 Mar-Apr;71(2):280-2.
A study was conducted to determine the accuracy and precision of 3 AOAC methods, secs 26.026-26.031 (CB), secs 26.032-26.036 (BF), and secs 26.052-26.060 (cottonseed), and the Romer quantitative method for the thin-layer chromatographic (TLC) determination of aflatoxins B1, B2, G1, and G2 in raisins. The samples were spiked at a level of 10 micrograms total aflatoxins/kg. The TLC development systems were: ether-methanol-water (94 + 4.5 + 1.5) and chloroform-acetone (9 + 1). The interaction between the 4 methods and the 2 development systems was also studied. The average recoveries were 88, 80, 75, and 93% with coefficients of variation of 14.0, 10.4, 14.0, and 9.6% for aflatoxin B1 using the CB, BF, cottonseed, and Romer methods, respectively. Statistical analysis showed no difference in the results obtained using the 2 TLC development systems.
开展了一项研究，以确定3种美国官方分析化学师协会（AOAC）方法（第26.026 - 26.031节（CB）、第26.032 - 26.036节（BF）和第26.052 - 26.060节（棉籽））以及罗默定量法对葡萄干中黄曲霉毒素B1、B2、G1和G2进行薄层色谱（TLC）测定的准确性和精密度。样品中黄曲霉毒素总量的添加水平为10微克/千克。TLC展开系统为：乙醚 - 甲醇 - 水（94 + 4.5 + 1.5）和氯仿 - 丙酮（9 + 1）。还研究了这4种方法与2种展开系统之间的相互作用。使用CB法、BF法、棉籽法和罗默法测定黄曲霉毒素B1时，平均回收率分别为88%、80%、75%和93%，变异系数分别为14.0%、10.4%、14.0%和9.6%。统计分析表明，使用2种TLC展开系统得到的结果没有差异。
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