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同时薄层色谱法测定黑橄榄中的黄曲霉毒素B1和赭曲霉毒素A

Simultaneous thin layer chromatographic determination of aflatoxin B1 and ochratoxin A in black olives.

作者信息

Le Tutour B, Tantaoui-Elaraki A, Aboussalim A

出版信息

J Assoc Off Anal Chem. 1984 May-Jun;67(3):611-2.

PMID:6430866
Abstract

A screening method has been developed for simultaneous determination of aflatoxin B1 and ochratoxin A in black olives. The technique includes extraction of both mycotoxins with aqueous methanol, cleanup using lead acetate, defatting with hexane, partitioning in chloroform, and thin layer chromatography. Detection limits are 5-7 micrograms aflatoxin B1 and 20 micrograms ochratoxin A/kg.

摘要

已开发出一种用于同时测定黑橄榄中黄曲霉毒素B1和赭曲霉毒素A的筛查方法。该技术包括用甲醇水溶液提取两种霉菌毒素,用醋酸铅净化,用己烷脱脂,在氯仿中分配,以及薄层色谱法。黄曲霉毒素B1的检测限为5 - 7微克,赭曲霉毒素A的检测限为20微克/千克。

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