Department of Gynaecology, Ganzhou People's Hospital of Jiangxi Province, No. 18, Meiguan Avenue, Ganzhou city, 341000 Jiangxi Province China.
Cell Mol Biol Lett. 2019 Jul 10;24:49. doi: 10.1186/s11658-019-0172-y. eCollection 2019.
has been demonstrated to be highly expressed in various human cancers including cervical cancer, and has been shown to play a critical role in tumor aggressiveness. We aimed to investigate the role of in human cervical cancer in vitro and in vivo.
Reverse transcription-quantitative polymerase chain reaction assay and Western blot assay were used to detect the mRNA and protein expression, respectively. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and were performed to measure the viabilities of cancer cells. Flow cytometry assay was used to detect the cell cycle and apoptosis. Moreover, an animal experiment was performed to evaluate the biological behavior of in vivo.
In the current study, we found that was obviously over-expressed in cervical cancer tissues compared to the adjacent non-cancer tissues. Cervical cancer cells transfected with siRNA demonstrated significant inhibition of cancer proliferation ( < 0.01), cell cycle arrest at G0/G1 phase, and cell apoptosis ( < 0.05). Moreover, down-regulation of significantly inhibited cervical cancer growth in vivo. In addition, protein levels of , c-Myc and Cyclin D1 were much lower in the siRNA-treated groups than that in the control group.
inhibition reduced cervical cancer tumor growth through the AKT pathway. This effect represented a therapeutic opportunity and provided a novel target for cervical cancer treatment.
已被证明在包括宫颈癌在内的多种人类癌症中高度表达，并被证明在肿瘤侵袭性中发挥关键作用。我们旨在研究在体外和体内中对人类宫颈癌的作用。
逆转录定量聚合酶链反应检测和 Western blot 检测分别用于检测 mRNA 和蛋白质表达。3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐法和集落形成实验用于测量癌细胞的活力。流式细胞术用于检测细胞周期和细胞凋亡。此外，进行动物实验以评估在体内的生物学行为。
在本研究中，我们发现与相邻非癌组织相比，在宫颈癌组织中明显过表达。转染的宫颈癌细胞 siRNA 显示出对癌症增殖的显著抑制（<0.01），G0/G1 期细胞周期停滞和细胞凋亡（<0.05）。此外，下调显著抑制了体内宫颈癌的生长。此外，在 siRNA 处理组中的、c-Myc 和 Cyclin D1 的蛋白水平明显低于对照组。
抑制通过 AKT 通路减少宫颈癌肿瘤生长。这种作用代表了一种治疗机会，并为宫颈癌治疗提供了新的靶标。
