用丁酸4-甲基伞形酮酯快速鉴定卡他布兰汉菌。
Rapid identification of Branhamella catarrhalis with 4-methylumbelliferyl butyrate.
作者信息
Vaneechoutte M, Verschraegen G, Claeys G, Flamen P
机构信息
Department of Medical Microbiology, University Hospital, Ghent, Belgium.
出版信息
J Clin Microbiol. 1988 Jun;26(6):1227-8. doi: 10.1128/jcm.26.6.1227-1228.1988.
Abstract
Branhamella catarrhalis can be distinguished from Neisseria spp. by the presence of butyrate esterase. This enzyme can be rapidly detected when 4-methylumbelliferyl butyrate is used as the substrate. All B. catarrhalis strains tested gave a positive fluorescence reaction within 5 min, while Neisseria spp. remained negative, even after 18 h of incubation.
摘要
卡他布兰汉菌可通过丁酸酯酶的存在与奈瑟菌属相区分。当以4-甲基伞形酮丁酸酯作为底物时，这种酶能够被快速检测到。所有测试的卡他布兰汉菌菌株在5分钟内均给出阳性荧光反应，而奈瑟菌属即便在培养18小时后仍为阴性。
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