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B.CAT确认试验,一种用于确认卡他布兰汉菌的快速检测方法。

B.CAT CONFIRM, a rapid test for confirmation of Branhamella catarrhalis.

作者信息

Janda W M, Ruther P

机构信息

Department of Medical Laboratory Sciences, College of Associated Health Professions, University of Illinois 60612.

出版信息

J Clin Microbiol. 1989 May;27(5):1130-1. doi: 10.1128/jcm.27.5.1130-1131.1989.

Abstract

B.CAT CONFIRM (Scott Laboratories, Inc., Fiskeville, R.I.), a rapid test for detection of tributyrin hydrolysis, was evaluated for its ability to identify strains of Branhamella catarrhalis and to differentiate them from Neisseria species and related species. On initial testing, B.CAT CONFIRM was positive for 65 (96%) of 68 B. catarrhalis strains within 30 min after inoculation. Retesting of the remaining three strains resulted in their correct identification. B.CAT CONFIRM was negative for all Neisseria spp. (130 strains) and for Kingella spp. (3 strains). Two of the three Moraxella spp. were weakly positive in the B.CAT CONFIRM after 60 min, but these reactions were easily distinguishable from the strong reactions of B. catarrhalis strains. This test will be helpful in the clinical laboratory for the rapid identification of B. catarrhalis in clinical specimens.

摘要

B.CAT CONFIRM(斯科特实验室公司,罗德岛州菲斯克维尔)是一种用于检测三丁酸甘油酯水解的快速检测方法,对其鉴定卡他布兰汉菌菌株并将其与奈瑟菌属及相关菌属进行区分的能力进行了评估。在初次检测中,68株卡他布兰汉菌菌株接种后30分钟内,有65株(96%)通过B.CAT CONFIRM检测呈阳性。对其余3株进行重新检测后得以正确鉴定。所有奈瑟菌属菌株(130株)和金氏菌属菌株(3株)通过B.CAT CONFIRM检测均为阴性。3株莫拉菌属菌株中有2株在60分钟后通过B.CAT CONFIRM检测呈弱阳性,但这些反应与卡他布兰汉菌菌株的强阳性反应易于区分。该检测方法将有助于临床实验室快速鉴定临床标本中的卡他布兰汉菌。

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