Ennis P D, Jackson A P, Parham P
Department of Cell Biology, Stanford University School of Medicine, CA 94305.
J Immunol. 1988 Jul 15;141(2):642-51.
Two cDNA cloned from a Hereford cow B cell line (BL-3) have allowed the determination of the complete coding region for two class I molecules encoded by the bovine MHC (BoLA). The predicted protein sequences have all the features expected of expressed class I molecules that present peptide Ag to cytotoxic T cells. Comparison with class I molecules from other species strongly suggests these cDNA are derived from different genes and provides evidence for the existence of a second expressed class I BoLA locus. The BoLA proteins show greater similarity to HLA than to H-2 molecules, correlating with the cross-reactions of W6/32 and other murine anti-HLA-A,B,C mAb with BoLA molecules. The basis for the W6/32 epitope and the preferential association of H-2 class I H chains with bovine beta 2-m is examined.
从一头赫里福德母牛的B细胞系（BL-3）克隆出的两个cDNA，使得确定牛MHC（BoLA）编码的两个I类分子的完整编码区成为可能。预测的蛋白质序列具有向细胞毒性T细胞呈递肽抗原的表达I类分子所预期的所有特征。与其他物种的I类分子比较强烈表明这些cDNA源自不同基因，并为第二个表达的I类BoLA基因座的存在提供了证据。BoLA蛋白与HLA的相似性高于与H-2分子的相似性，这与W6/32和其他鼠抗HLA-A、B、C单克隆抗体与BoLA分子的交叉反应相关。研究了W6/32表位的基础以及H-2 I类重链与牛β2-微球蛋白的优先结合。
