Lemonnier F A, Le Bouteiller P P, Malissen B, Golstein P, Malissen M, Mishal Z, Caillol D H, Jordan B R, Kourilsky F M
J Immunol. 1983 Mar;130(3):1432-8.
Murine LMTK- cells were unexpectedly found to cross-react with a murine anti-human beta 2-microglobulin (beta 2-m)monoclonal antibody (m.Ab) after transformation with cosmid clones containing different purified HLA class I genes. The same cross-reactivity was observed with CTP 34 B4 (murine x human) somatic hybrid cells, which express class I molecules constituted of human HLA heavy chains and murine beta 2-m. Inhibition studies of the complement-dependent cytolysis mediated by the cross-reacting m.Ab indicated that isolated murine beta 2-m does not express the cross-reacting determinant, suggesting that its expression by the transformed cells reflects conformational modification of murine beta 2-m upon its association with HLA heavy chains. These results illustrate one of the possible post-translational mechanisms through which the antigenicity of a polypeptide chain can be modified. They might provide a serologic marker of the third domain of HLA class I heavy chains. Finally, because quantitative differences of reactivity with the anti-human beta 2-m m.Ab were observed, depending on the HLA class I genes used for transformation, these results individualize two families of HLA class I heavy chains responsible for different conformational modifications of murine beta 2-m.
在用含有不同纯化HLA I类基因的黏粒克隆进行转化后，意外发现鼠源LMTK-细胞能与鼠抗人β2-微球蛋白（β2-m）单克隆抗体（m.Ab）发生交叉反应。在用表达由人HLA重链和鼠源β2-m构成的I类分子的CTP 34 B4（鼠×人）体细胞杂交细胞中也观察到了同样的交叉反应。对由交叉反应性m.Ab介导的补体依赖性细胞溶解的抑制研究表明，分离出的鼠源β2-m不表达交叉反应决定簇，这表明转化细胞中其表达反映了鼠源β2-m与HLA重链结合后构象的改变。这些结果说明了多肽链抗原性可被修饰的一种可能的翻译后机制。它们可能提供了HLA I类重链第三结构域的血清学标志物。最后，由于观察到与抗人β2-m m.Ab反应性的定量差异取决于用于转化的HLA I类基因，这些结果区分出了导致鼠源β2-m不同构象改变的两个HLA I类重链家族。
