Mecham J O, Dean V C
Agricultural Research Service, Arthropod-borne Animal Diseases Research Laboratory, University Station, Laramie, Wyoming 82071-3965.
J Gen Virol. 1988 Jun;69 ( Pt 6):1255-62. doi: 10.1099/0022-1317-69-6-1255.
Viral genomic RNA was purified from BHK-21 cells infected with epizootic haemorrhagic disease virus and the 10 dsRNA genome segments were isolated by polyacrylamide gel electrophoresis. These genome segments were translated in vitro using the rabbit reticulocyte lysate system and the synthesized proteins were detected by immune precipitation and gel electrophoresis. This allowed the assignment of protein coding to the genome segments and the identification of two additional virus-specified proteins not readily detectable in lysates of virus-infected cells.
从感染流行性出血病病毒的BHK - 21细胞中纯化病毒基因组RNA，并通过聚丙烯酰胺凝胶电泳分离出10个双链RNA基因组片段。使用兔网织红细胞裂解物系统对这些基因组片段进行体外翻译，通过免疫沉淀和凝胶电泳检测合成的蛋白质。这使得能够将蛋白质编码分配到基因组片段，并鉴定出在病毒感染细胞裂解物中不易检测到的另外两种病毒特异性蛋白质。
