Effect of autoclaving on the mechanical properties and structure of the giant squid hydrolysate-polysaccharide gel.

Fish hydrolysates are an attractive option for preparing composite gel foods. However, not much is known regarding their gel properties. Here, we investigated the effect of the autoclaving treatment (121°C, 10 min) on the microstructure and properties of the giant squid hydrolysate-konjac glucomannan-κ-carrageenan-locust bean gum gel (GSH-P gel). The nuclear magnetic resonance proton spin-spin relaxation time (T ) measurements indicated that autoclaving led to stronger water-binding ability of the GSH-P gel. The rheological measurements indicated that autoclaving led to significantly higher viscoelastic modulus and gel strength. The results of confocal laser scanning microscopy and small-angle X-ray scattering indicated that the phase separation of polysaccharides and proteins/peptides was enhanced by autoclaving, and the polysaccharides swelled better, resulting in less, but ordered, network structures. Autoclaving had a similar, but insignificant, positive effect on the polysaccharide (P) gel. Thus, GSH seems to play an important role in the process of polysaccharide gelation. This study shows that autoclaving can alter the structure and improve the properties of the GSH-P gel.