State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Key Laboratory of Tea Quality and Safety Control, Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China.
J Insect Physiol. 2019 Aug-Sep;117:103915. doi: 10.1016/j.jinsphys.2019.103915. Epub 2019 Jul 20.
Insect odorant binding proteins (OBPs), one of the most important groups of odor carriers, are believed to play essential roles in chemoreception. In the present study, we focused on AfasOBP11 in Adelphocoris fasciaticollis. Expression profiles showed that AfasOBP11 was mainly expressed in the mouthparts of A. fasciaticollis. Additionally, two types of sensilla, sensilla trichodeum and sensilla basiconicum, were found on the mouthparts of bugs. Moreover, anti-AfasOBP11 antiserum strongly labeled the sensilla basiconica. In fluorescence binding assays, recombinant AfasOBP11 displayed much stronger binding abilities to non-volatile secondary metabolite compounds than to volatile odors, suggesting a role of AfasOBP11 in taste sensing. To further investigate the biological functions of AfasOBP11, the feeding behavior of wild-type, dsGFP-injected and dsAfasOBP11-injected bugs was evaluated by performing electrical penetration graph (EPG) tests. After RNA interference of target AfasOBP11, A. fasciaticollis bugs spent a longer time and pierced more frequently on the artificial diet containing 2.0% gossypol, indicating that RNAi treated bugs reduced sensitivity to gossypol. Our findings suggest that AfasOBP11 may play a vital role in chemoreception of A. fasciaticollis, especially in gustatory perception.
昆虫气味结合蛋白（OBPs）是最重要的气味载体之一，被认为在化学感受中发挥着重要作用。本研究集中于东亚飞蝗（Adelphocoris fasciaticollis）的 AfasOBP11。表达谱显示，AfasOBP11 主要在东亚飞蝗的口器中表达。此外，在昆虫的口器上发现了两种感器，即毛形感器和刺形感器。并且，抗 AfasOBP11 抗血清强烈标记了刺形感器。在荧光结合测定中，重组 AfasOBP11 对非挥发性次生代谢化合物的结合能力明显强于对挥发性气味的结合能力，表明 AfasOBP11 在味觉感知中发挥作用。为了进一步研究 AfasOBP11 的生物学功能，通过进行电穿透图（EPG）测试来评估野生型、dsGFP 注射和 dsAfasOBP11 注射昆虫的取食行为。在靶标 AfasOBP11 的 RNA 干扰后，东亚飞蝗在含有 2.0%棉酚的人工饲料上花费的时间更长，穿孔更频繁，表明 RNAi 处理的昆虫对棉酚的敏感性降低。我们的研究结果表明，AfasOBP11 可能在东亚飞蝗的化学感受中发挥重要作用，尤其是在味觉感知中。
