State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
J Agric Food Chem. 2021 Jun 23;69(24):6769-6778. doi: 10.1021/acs.jafc.1c01319. Epub 2021 Jun 11.
Sex pheromones are deemed to play a significant role in sexual communication of most insects. Although many sex pheromone components in mirid bugs have been identified, the roles of odorant receptors in sex pheromone perception in (Hemiptera: Miridae) remain unknown so far. Here, AlinOR33, a candidate sex pheromone receptor in was functionally characterized. Phylogenetic analysis showed that AlinOR33 clustered with the sex pheromone receptor AlucOR4 from. Quantitative real-time PCR measurement revealed that the expression of increased gradually from nymph to adult stage and reached its peak in the antennae of 3-day-old mated male bugs. The subsequent hybridization demonstrated that 33 was mainly expressed in sensilla trichoid on the antennae of . In the two-electrode voltage clamp recordings, AlinOR33/AlinOrco was specifically tuned to four sex pheromone components including butyl butyrate, hexyl hexanoate, -2-hexenyl butyrate and hexyl butyrate, and especially most sensitive to the major component -2-hexenyl butyrate. After ds injection, the electroantennogram responses of males to four sex pheromone components were reduced significantly (∼50%). Compared to control bugs, ds-injected male bugs almost lost behavioral preference for -2-hexenyl butyrate. Furthermore, the wingbeat frequency of ds-injected male bugs notably declined. Therefore, we conclude that as a candidate sex pheromone receptor, AlinOR33 plays essential roles in the sexual behavior of .
性信息素被认为在大多数昆虫的性通讯中发挥重要作用。尽管已经鉴定出许多盲蝽科昆虫的性信息素成分，但性信息素感受在（半翅目：盲蝽科）中的作用仍然未知。在这里，（Hemiptera: Miridae）中的候选性信息素受体 AlinOR33 被功能表征。系统发育分析表明，AlinOR33 与来自的性信息素受体 AlucOR4 聚类。定量实时 PCR 测量显示，从若虫到成虫阶段，的表达逐渐增加，并在 3 天龄交配雄性虫的触角中达到峰值。随后的杂交表明，主要表达在触角的毛状感器上。在双电极电压钳记录中，AlinOR33/AlinOrco 特异性调谐到包括丁酸丁酯、己酸己酯、丁酸-2-己烯酯和丁酸己酯在内的四种性信息素成分，特别是对主要成分丁酸-2-己烯酯最敏感。ds 注射后，雄性对四种性信息素成分的触角电生理反应显著降低（约 50%）。与对照虫相比，ds 注射的雄性虫对丁酸-2-己烯酯的行为偏好几乎丧失。此外，ds 注射的雄性虫的翅膀拍打频率明显下降。因此，我们得出结论，作为候选性信息素受体，AlinOR33 在的性行为中发挥重要作用。
