Nutraceutical polyphenols: New analytical challenges and opportunities.

Nowadays, the research for secondary metabolites with health promoting effects in countering or slowing-down chronic and degenerative diseases (e.g. cancer, cardiovascular, and neurodegenerative diseases) identify phenols and polyphenols, widespread and mostly copious in dietary plant sources, as beneficial for human health. These compounds, as intrinsically antioxidant, are claimed as nutraceuticals with preventive efficacy in offsetting oxidant species over-genesis in normal cells, and with the potential ability to halt or reverse oxidative stress-related diseases. In this context, pure (poly)phenols and/or their herbal/food complexes were found to exert both anti- and pro-oxidant activities, suggesting also a promising chemopreventive efficacy. In fact, different evidence further highlights their ability to induce apoptosis, growth arrest, DNA synthesis inhibition and/or modulation of signal transduction pathways. Indeed, a full understanding of the phenolic and polyphenolic composition of plant species, which still now represent their inestimable and worth exploring source, is an important challenge, which today can and must be favourably pursued in the consciousness that the bioactivity of a plant extract is always in its chemistry. To reach this purpose a number of new and advanced techniques are available for extraction, purification and structural identification purposes, but, taking into account how, when and where (poly)phenols are biosynthesized, their use must be highly rationalized. This is particularly true for mass spectrometry techniques which, although representing one of the most powerful tools and in continuous evolution in this era, often suffer from an automatism that does not give justice to the chemical goodness of a plant species and particularly those of nutraceutical interest. This review will deepen into polyphenol research, focusing on biosynthesis, analytical approaches for a conscious exploitability of nutraceutical plant extracts rich in antioxidant and anti-inflammatory polyphenols and/or pure isolated polyphenols.