Abualzulof Ghaid W A, Scandar Samir, Varfaj Ina, Dalla Costa Vanessa, Sardella Roccaldo, Filippini Raffaella, Piovan Anna, Marcotullio Maria Carla
Department of Pharmaceutical Sciences, University of Perugia, Via Fabretti 48, 06123 Perugia, Italy.
Department of Pharmaceutical Sciences, University of Padua, Via Marzolo, 5, 35131 Padua, Italy.
Antioxidants (Basel). 2024 Sep 18;13(9):1129. doi: 10.3390/antiox13091129.
spp. are often used as food and in traditional medicine, and their biological activities as anti-inflammatory and diuretic, for wound healing, and as antimicrobial agents have been largely reviewed. The aim of this work was to investigate the polyphenol content and the antioxidant and anti-tyrosinase properties of the extracts from , a very poorly explored species. For this purpose, leaves were collected at three different maturity stages (H1, H2, and H3), and the environmentally sustainable methanolic extracts were evaluated for the total phenolic content (TPC), total flavonoid content (TFC), and total catechins content (TCC). The polyphenolic profile was studied using HPLC-UV/DAD and UHPLC-MS, and the antioxidant activity was determined in vitro using DPPH, FRAP, and ABTS assays. The study showed that the H2 extract had higher TPC and TFC values (113.50 mg GA/g and 43.27 mg QE/g, respectively) and significant antioxidant activity. Therefore, the H2 extract was selected to study the anti-tyrosinase activity. The results also showed that H2 was able to bind and inhibit tyrosinase, with rutin being the compound responsible for the measured activity on the enzyme.
某些物种常被用作食物和传统药物，其抗炎、利尿、促进伤口愈合及抗菌等生物活性已得到大量综述。本研究的目的是调查一种研究极少的物种的提取物中的多酚含量、抗氧化和抗酪氨酸酶特性。为此，在三个不同成熟阶段（H1、H2和H3）采集了该物种的叶子，并对环境可持续的甲醇提取物进行了总酚含量（TPC）、总黄酮含量（TFC）和总儿茶素含量（TCC）的评估。使用HPLC-UV/DAD和UHPLC-MS研究了多酚谱，并使用DPPH、FRAP和ABTS测定法在体外测定了抗氧化活性。研究表明，H2提取物具有较高的TPC和TFC值（分别为113.50 mg GA/g和43.27 mg QE/g）以及显著的抗氧化活性。因此，选择H2提取物来研究抗酪氨酸酶活性。结果还表明，H2能够结合并抑制酪氨酸酶，芦丁是对该酶测得活性负责的化合物。
