Robidoux C, Maclouf J, Pradelles P, Sirois P
Dép. Pharmacologie, Faculté de Médecine, Université de Sherbrooke, P.Q., Canada.
Prostaglandins Leukot Essent Fatty Acids. 1988 Apr;32(1):23-8. doi: 10.1016/0952-3278(88)90089-0.
Human lung specimens were minced and treated for 30 min with collagenase (1 mg ml-1) and DNase (0.1 mg ml-1) to obtain a suspension of viable (approximately 80%) and metabolically active lung cells (5 x 10(6) cells per gram of tissue). Treatment of these mixed lung cells with bradykinin (1.25 x 10(-6) to 1 x 10(-5) M) and f-Met-Leu-Phe (f-MLP; 1 x 10(-8) to 5 x 10(-6) M) did not stimulate to a substantial extent the release of prostaglandins and thromboxanes (measured with novel Enzyme Immunoassays). The only concentration of PAF that stimulated significantly the release of icosanoids from lung cells was 5 x 10(-7) M. Phorbol myristate (PMA; 5 x 10(-8) to 2 x 10(-6) M) and ionophore a-21387 (2.5 x 10(-6) to 2 x 10(-5) M) strongly stimulated the release of prostaglandins and thromboxanes by dispersed human lung cells. These findings support previous observations showing that human lungs have the enzymes necessary for the synthesis and release of prostaglandins and thromboxanes but stimulation of the release of these mediators is not obtained with the hormonal stimuli that are active in guinea pigs. Studies in progress will purify the cell populations and characterize the cells responsible for the release of these icosanoids.
将人肺组织标本切碎，用胶原酶（1毫克/毫升）和脱氧核糖核酸酶（0.1毫克/毫升）处理30分钟，以获得有活力（约80%）且代谢活跃的肺细胞悬液（每克组织含5×10⁶个细胞）。用缓激肽（1.25×10⁻⁶至1×10⁻⁵摩尔/升）和N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（f-MLP；1×10⁻⁸至5×10⁻⁶摩尔/升）处理这些混合肺细胞，并未在很大程度上刺激前列腺素和血栓烷的释放（用新型酶免疫测定法测量）。能显著刺激肺细胞释放类二十烷酸的PAF唯一浓度是5×10⁻⁷摩尔/升。佛波醇肉豆蔻酸酯（PMA；5×10⁻⁸至2×10⁻⁶摩尔/升）和离子载体A-23187（2.5×10⁻⁶至2×10⁻⁵摩尔/升）强烈刺激分散的人肺细胞释放前列腺素和血栓烷。这些发现支持了先前的观察结果，即人肺具有合成和释放前列腺素及血栓烷所需的酶，但这些介质的释放不会因在豚鼠中起作用的激素刺激而得到激发。正在进行的研究将纯化细胞群体，并鉴定负责释放这些类二十烷酸的细胞。
