Robidoux C, Pelé J P, Maclouf J, Pradelles P, Sirois P
Département de Pharmacologie, Faculté de Médecine, Université de Sherbrooke, P.Q., Canada.
Inflammation. 1988 Aug;12(4):285-95. doi: 10.1007/BF00915766.
Guinea pig lungs were perfused for 15 min with a solution of protease type VII (0.05 mg/ml) and dispersed to yield a suspension of morphologically intact and metabolically active lung cells (over 250 X 10(6) cells per animal). The viability of these cells assessed with the Trypan blue exclusion technique was more than 80%. Treatment of these mixed cells (1 X 10(6) cells/ml) with chemicals such as phorbol myristate acetate (1 X 10(-9) to 1 X 10(-7) M), f-Met-Leu-Phe (5 X 10(-6) to 1 X 10(-4) M), and the calcium ionophore A23187 (3.1 X 10(-7) to 2.5 X 10(-6) M), and with autacoids such as bradykinin (1 X 10(-5) to 1 X 10(-4) M), leukotriene B4 (1 X 10(-13) to 1 X 10(-7) M), and leukotriene D4 (1 X 10(-10) to 1 X 10(-7) M) stimulated to a variable degree the release of prostaglandin E2 and thromboxane B2 (measured with a novel enzyme immunoassay). It is suggested that icosanoid release from the lungs is the result of direct chemical or hormonal stimulation of the cells and not a consequence of vascular changes. Studies are in progress to purify lung cell populations and characterize the cells responsible for the release of these icosanoids.
用VII型蛋白酶溶液（0.05毫克/毫升）对豚鼠肺进行15分钟灌注，然后分散以获得形态完整且代谢活跃的肺细胞悬液（每只动物超过250×10⁶个细胞）。用台盼蓝排斥技术评估这些细胞的活力超过80%。用化学物质如佛波酯肉豆蔻酸乙酸酯（1×10⁻⁹至1×10⁻⁷M）、f - 甲硫氨酸 - 亮氨酸 - 苯丙氨酸（5×10⁻⁶至1×10⁻⁴M）和钙离子载体A23187（3.1×10⁻⁷至2.5×10⁻⁶M），以及用自分泌物质如缓激肽（1×10⁻⁵至1×10⁻⁴M）、白三烯B4（1×10⁻¹³至1×10⁻⁷M）和白三烯D4（1×10⁻¹⁰至1×10⁻⁷M）处理这些混合细胞（1×10⁶个细胞/毫升），不同程度地刺激了前列腺素E2和血栓素B2的释放（用一种新型酶免疫测定法测量）。提示肺中类二十烷酸的释放是细胞受到直接化学或激素刺激的结果，而非血管变化的后果。目前正在进行研究以纯化肺细胞群体并鉴定负责释放这些类二十烷酸的细胞。
