Carlos Chagas Filho Institute of Biophysics and National Center for Structural Biology and Bioimaging/CENABIO, Federal University of Rio de Janeiro, Rio de Janeiro 21941-102, Brazil.
Laboratory of Proteomics, LADETEC, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro 21941-598, Brazil.
Cells. 2019 Jul 11;8(7):703. doi: 10.3390/cells8070703.
Omics approaches have significantly impacted knowledge about molecular signaling pathways driving cell function. Induced pluripotent stem cells (iPSC) have revolutionized the field of biological sciences and proteomics and, in particular, has been instrumental in identifying key elements operating during the maintenance of the pluripotent state and the differentiation process to the diverse cell types that form organisms. This review covers the evolution of conceptual and methodological strategies in proteomics; briefly describes the generation of iPSC from a historical perspective, the state-of-the-art of iPSC-based proteomics; and compares data on the proteome and transcriptome of iPSC to that of embryonic stem cells (ESC). Finally, proteomics of healthy and diseased cells and organoids differentiated from iPSC are analyzed.
组学方法极大地影响了对驱动细胞功能的分子信号通路的认识。诱导多能干细胞 (iPSC) 彻底改变了生物科学和蛋白质组学领域，特别是在鉴定维持多能状态和分化过程中关键元素方面发挥了重要作用，分化过程产生了形成生物体的各种细胞类型。本综述涵盖了蛋白质组学中概念和方法策略的演变；简要描述了从历史角度看 iPSC 的产生，基于 iPSC 的蛋白质组学的最新进展；并比较了 iPSC 和胚胎干细胞 (ESC) 的蛋白质组和转录组数据。最后，分析了 iPSC 分化的健康细胞和类器官的蛋白质组学。
