Key Laboratory of Forage and Endemic Crop Biotechnology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China.
Genes (Basel). 2019 Jul 12;10(7):526. doi: 10.3390/genes10070526.
RNAi (RNA interference) is an important defense response against virus infection in plants. The core machinery of the RNAi pathway in plants include DCL (Dicer Like), AGO (Argonaute) and RdRp (RNA dependent RNA polymerase). Although involvement of these RNAi components in virus infection responses was demonstrated in , their contribution to antiviral immunity in , a model plant for plant-pathogen interaction studies, is not well understood. In this study, we investigated the role of gene against TMV (Tomato mosaic virus) infection. Silencing of by transient expression of an hpRNA construct recovered GFP (Green fluorescent protein) expression in -silenced plant, demonstrating that participated in RNAi process in . Expression of was transcriptionally induced by both MeSA (Methylsalicylate acid) treatment and TMV infection. Down-regulation of gene by amiR- transient expression compromised plant resistance against TMV infection. Inhibition of endogenous miR403a, a predicted regulatory microRNA of , reduced TMV infection. Our study provides evidence for the antiviral role of against a family virus TMV in , and SA (Salicylic acid) mediates this by induction of expression upon TMV infection. Our data also highlighted that miR403a was involved in TMV defense by regulation of target gene in .
RNAi（RNA 干扰）是植物抵御病毒感染的重要防御反应。植物中 RNAi 途径的核心机制包括 DCL（Dicer-like）、AGO（Argonaute）和 RdRp（RNA 依赖的 RNA 聚合酶）。尽管这些 RNAi 成分在病毒感染反应中的参与已在 中得到证实，但它们在 中抗病毒免疫的贡献尚不清楚， 是植物-病原体相互作用研究的模式植物。在本研究中，我们研究了 基因对 TMV（番茄花叶病毒）感染的作用。通过瞬时表达 hpRNA 构建体沉默 ，恢复了 -沉默植物中的 GFP（绿色荧光蛋白）表达，证明 参与了 中的 RNAi 过程。MeSA（甲基水杨酸）处理和 TMV 感染均诱导 的转录表达。通过 amiR-瞬时表达下调 基因会削弱植物对 TMV 感染的抗性。抑制内源性 miR403a，一种 的预测调控 microRNA，可降低 TMV 感染。我们的研究为 中的 基因针对 TMV 家族病毒的抗病毒作用提供了证据，而 SA（水杨酸）通过 TMV 感染诱导 的表达来介导这一作用。我们的数据还强调了 miR403a 通过调控靶标 基因参与 TMV 防御。
