College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China.
Int J Mol Sci. 2019 Jul 13;20(14):3452. doi: 10.3390/ijms20143452.
Histone acetylation and deacetylation play essential roles in eukaryotic gene regulation. HD2 (HD-tuins) proteins were previously identified as plant-specific histone deacetylases. In this study, we investigated the function of the gene in the formation of stem vascular tissue in . The height and thickness of the inflorescence stems in the mutant was lower than that of wild-type plants. Paraffin sections showed that the cell number increased compared to the wild type, while transmission electron microscopy showed that the size of individual tracheary elements and fiber cells significantly decreased in the mutant. In addition, the cell wall thickness of tracheary elements and fiber cells increased. We also found that the lignin content in the stem of the mutants increased compared to that of the wild type. Transcriptomic data revealed that the expression levels of many biosynthetic genes related to secondary wall components, including cellulose, lignin biosynthesis, and hormone-related genes, were altered, which may lead to the altered phenotype in vascular tissue of the mutant. These results suggested that is involved in development of the vascular tissue of the stem by affecting cell proliferation and differentiation.
组蛋白乙酰化和去乙酰化在真核基因调控中发挥着重要作用。HD2（HD-tuins）蛋白先前被鉴定为植物特异性组蛋白去乙酰化酶。在这项研究中，我们研究了 基因在 茎维管组织形成中的功能。 突变体的花序茎的高度和厚度比野生型植物低。石蜡切片显示与野生型相比细胞数量增加，而透射电子显微镜显示 突变体中单个导管元件和纤维细胞的大小显著减小。此外，导管元件和纤维细胞的细胞壁厚度增加。我们还发现， 突变体茎中的木质素含量比野生型增加。转录组数据显示，与次生壁成分（包括纤维素、木质素生物合成和激素相关基因）相关的许多生物合成基因的表达水平发生了改变，这可能导致 突变体维管束组织表型的改变。这些结果表明， 通过影响细胞增殖和分化参与茎维管组织的发育。
