Nanoscience Laboratory, Department of Physics, University of Trento, 38123 Trento, Italy.
LaBSSAH, Fondazione Bruno Kessler, 38123 Trento, Italy.
Toxins (Basel). 2019 Jul 14;11(7):409. doi: 10.3390/toxins11070409.
Aflatoxins (AF) are naturally occurring mycotoxins, produced by many species of Aspergillus. Among aflatoxins, Aflatoxin M1 (AFM1) is one of the most frequent and dangerous for human health. The acceptable maximum level of AFM1 in milk according to EU regulation is 50 ppt, equivalent to 152 pM, and 25 ppt, equivalent to 76 pM, for adults and infants, respectively. Here, we study a photonic biosensor based on Si 3 N 4 asymmetric Mach-Zehnder Interferometers (aMZI) functionalized with Fab' for AFM1 detection in milk samples (eluates). The minimum concentration of AFM1 detected by our aMZI sensors is 48 pM (16.8 pg/mL) in purified and concentrated milk samples. Moreover, the real-time detection of the ligand-analyte binding enables the study of the kinetics of the reaction. We measured the kinetic rate constants of the Fab'-AFM1 interaction.
黄曲霉毒素（AF）是一种天然存在的真菌毒素，由许多曲霉属物种产生。在黄曲霉毒素中，黄曲霉毒素 M1（AFM1）对人类健康最常见且最危险。根据欧盟法规，牛奶中 AFM1 的可接受最大水平为 50 ppt，相当于 152 pM，成人和婴儿分别为 25 ppt，相当于 76 pM。在这里，我们研究了一种基于氮化硅不对称马赫-曾德尔干涉仪（aMZI）的光子生物传感器，该传感器用 Fab'进行功能化，用于检测牛奶样品（洗脱液）中的 AFM1。我们的 aMZI 传感器在纯化和浓缩的牛奶样品中检测到的 AFM1 的最低浓度为 48 pM（16.8 pg/mL）。此外，配体-分析物结合的实时检测使我们能够研究反应动力学。我们测量了 Fab'-AFM1 相互作用的动力学速率常数。
