Unravelling the Role of Trophoblastic-Derived Extracellular Vesicles in Regulatory T Cell Differentiation.

Regulatory T cells (T) are mandatory elements in the maintenance of human pregnancy, but their de novo differentiation has not been completely exposed. HSPE1 chaperone expressing trophoblast cells may have a role in it. Trophoblast-derived extracellular vesicles (EVs), either at the feto-maternal interface or in circulation, target CD4 T cells. We hypothesized that HSPE1-associated trophoblastic cell line (BeWo)-derived EVs are active mediators of T cell differentiation. We proved at first that recombinant HSPE1 promote human T cell differentiation in vitro. Developing a CRISPR-Cas9 based knockout BeWo cell line we could also demonstrate, that EV-associated HSPE1 induces T development. Next-generation sequencing of miRNA cargo of BeWo-EVs characterized the regulatory processes of T polarization. By the use of single-cell transcriptomics analysis, seven T cell subtypes were distinguished and we demonstrated for the first time that the expression level of was T subtype dependent, and expression is characteristic to memory phenotype of T cells. Our data indicate that and may be used as markers for identification of T subtypes. Our results suggest, that trophoblastic-derived iEVs-associated HSPE1 and miRNA cargo have an important role in T cell expansion in vitro and is a useful marker of T subtype characterization.