Kubesch P, Lingner M, Grothues D, Wehsling M, Tümmler B
Zentrum Biochemie II, Hanover Medical School, FRG.
Scand J Gastroenterol Suppl. 1988;143:77-80.
The early stage of the colonization of Pseudomonas aeruginosa in cystic fibrosis (CF) was investigated. Most CF patients, once they have become colonized, harboured genomically related P. aeruginosa strains in their respiratory tract over long periods. Unrelated CF patients were colonized with different strains; however, within families cross-infection between CF siblings frequently took place. Adherence tests with buccal epithelial cells demonstrated that the epithelial cell layer of the oropharynx remained intact for up to 2 years after the onset of colonization with P. aeruginosa. Among the constituents of the microcolony the bronchial mucins were determined to be the major binding targets of CF isolates of P. aeruginosa. The time course of antibody formation to outer membrane antigens of P. aeruginosa in CF serum was analysed by Western immunoblots. Lipopolysaccharide and protein H induced the first systemic immune response.
对囊性纤维化（CF）患者中铜绿假单胞菌的早期定植情况进行了研究。大多数CF患者一旦被定植，其呼吸道中长期携带基因组相关的铜绿假单胞菌菌株。无亲缘关系的CF患者被不同菌株定植；然而，在家庭中CF兄弟姐妹之间经常发生交叉感染。对颊上皮细胞的黏附试验表明，在用铜绿假单胞菌定植后长达2年的时间里，口咽部的上皮细胞层保持完整。在微菌落的成分中，支气管黏蛋白被确定为铜绿假单胞菌CF分离株的主要结合靶点。通过Western免疫印迹分析了CF血清中针对铜绿假单胞菌外膜抗原的抗体形成时间进程。脂多糖和蛋白H诱导了首次全身免疫反应。
