Kazemi-Darabadi Siamak, Asadpour Reza, Shahbazfar Amir Ali, Alizadeh Sajjad
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Vet Res Forum. 2019 Spring;10(2):125-132. doi: 10.30466/vrf.2019.74767.2002. Epub 2019 Jun 15.
Testicular torsion is a consequence of spermatic cord twisting which causes progressive damage to the structure of the testis and reduces sperm quality and usually results in infertility. In the present study, with the assumption of the protective effects of L-carnitine and betamethasone against ischemia-reperfusion (IR) injuries, their effects on twisted testicles were evaluated and compared. Twenty Wistar rats were randomly divided into four groups and used in this study. Except for the Sham (S) group, testicular IR was induced surgically in three other groups, including Control (C), Betamethasone (BM), and L-carnitine (LC) groups. Betamethasone and L-carnitine were injected before detorsion in the BM and LC groups, respectively. After twelve hours of reperfusion, the testicles were detached, and prepared for sperm parameters evaluation such as sperm count, motility, viability, morphology, and chromatin quality, and histopathologic evaluations, including mean seminiferous tubular diameter (MSTD), germinal epithelial cell thickness (GECT), and Johnsen's mean testicular biopsy scoring (MTBS). The MSTD, GECT, and healthy sperms in the C group were significantly lower than the other groups, while the BM and LC groups were significantly different from others in MTBS. The number of sperms and sperm motility in the BM group was significantly higher than the C group. Sperm viability in the BM and LC groups were significantly higher than the C group. The results of this study showed that both L-carnitine and betamethasone similarly can be effective in treating testicular IR injuries.
睾丸扭转是精索扭转的结果，会对睾丸结构造成渐进性损害，降低精子质量，通常导致不育。在本研究中，假设左旋肉碱和倍他米松对缺血再灌注（IR）损伤有保护作用，评估并比较了它们对扭转睾丸的影响。将20只Wistar大鼠随机分为四组用于本研究。除假手术（S）组外，其他三组包括对照组（C）、倍他米松（BM）组和左旋肉碱（LC）组均通过手术诱导睾丸IR。BM组和LC组分别在解除扭转前注射倍他米松和左旋肉碱。再灌注12小时后，分离睾丸，准备评估精子参数，如精子数量、活力、存活率、形态和染色质质量，以及进行组织病理学评估，包括生精小管平均直径（MSTD）、生精上皮细胞厚度（GECT）和约翰森平均睾丸活检评分（MTBS）。C组的MSTD、GECT和健康精子数量显著低于其他组，而BM组和LC组的MTBS与其他组有显著差异。BM组的精子数量和精子活力显著高于C组。BM组和LC组的精子存活率显著高于C组。本研究结果表明，左旋肉碱和倍他米松在治疗睾丸IR损伤方面同样有效。
