Taati Majid, Moghadasi Mehrnoush, Dezfoulian Omid, Asadian Peyman
Department of Pathobiology, School of Veterinary Medicine, Lorestan University, Khorramabad, Iran.
Department of Physiology, School of Medicine, Lorestan University of Medical Sciences, Khoramabad, Iran.
Acta Med Iran. 2016 Jan;54(1):32-8.
Ischemia-reperfusion injury is a possible cause of testicular damage and infertility after testicular torsion and detorsion. The purpose of this study was to evaluate the effect of ghrelin on testicular ischemia-reperfusion damage. A total of 30 adult male rats were selected for the study and divided randomly into 3 groups, each containing 10 rats. Animals in the testicular torsion and ghrelin treated groups were subjected to unilateral 720° counterclockwise testicular torsion for 1 hour, and then reperfusion was allowed after detorsion for 7 and 30 days. The ghrelin-treated group and the other two groups received intraperitoneally 40 nmol of ghrelin and physiological saline 15 min before detorsion, respectively. The animals were sacrificed at the end of reperfusion times, and their testes were taken for later histopathological examination. The seminiferous tubules diameter, germinal epithelium height, as well as volume densities in testicular torsion / detorsion plus saline group, were significantly lesser versus control group, which clearly indicates an ischemia-reperfusion injury. Ghrelin treatment resulted in a partial increment in examined histological parameters on day 30 after reperfusion. Current results showed that ghrelin ameliorates the testicular ischemia-reperfusion damage.
缺血再灌注损伤是睾丸扭转及扭转复位后睾丸损伤和不育的一个可能原因。本研究的目的是评估胃饥饿素对睾丸缺血再灌注损伤的影响。总共选取30只成年雄性大鼠进行研究，随机分为3组，每组10只。睾丸扭转加胃饥饿素治疗组的动物进行单侧720°逆时针睾丸扭转1小时，然后在扭转复位后7天和30天进行再灌注。胃饥饿素治疗组和其他两组分别在扭转复位前15分钟腹腔注射40 nmol胃饥饿素和生理盐水。在再灌注结束时处死动物，取出其睾丸用于后续的组织病理学检查。睾丸扭转/扭转复位加生理盐水组的生精小管直径、生精上皮高度以及体积密度与对照组相比明显更小，这清楚地表明存在缺血再灌注损伤。胃饥饿素治疗导致再灌注30天后所检测的组织学参数有部分增加。目前的结果表明，胃饥饿素可改善睾丸缺血再灌注损伤。
