Xia Zhaobo, Hu Jun, Han Lei, Xia Qiongzhang, Shao Fangbin, Lin Xiaokun
Department of Pediatric Surgery, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, Zhejiang, China.
Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, 650101, Yunnan, China.
Pediatr Surg Int. 2020 Apr;36(4):529-536. doi: 10.1007/s00383-020-04626-y. Epub 2020 Feb 18.
The aim of this study was to investigate the function of the contralateral testis after unilateral testicular torsion (UTT) and its possible mechanism.
56 rats were randomly divided into four groups: Group A: Sham operation, Group B: Testicular torsion (TT)+normal saline (NS), Group C: Testicular torsion (TT)+cyclosporine, Group D: Testicular torsion (TT)+NG-Monomethyl-L-arginine (L-NMMA). The right testes were removed 1 week and 8 weeks after surgery, respectively. Biochemistry and histopathologic evaluations were used to evaluate the germ cell damage.
Compared with Group A, the levels of malondialchehyche (MDA) and nitric oxide (NO)/nitricoxide synthase (NOS) were increased remarkably in Group B. Significant differences were shown between histopathological damages and density and motility of sperm in two groups. Compared with Group B, the levels of MDA and NO/NOS in Group D decreased significantly while mean seminiferous tubule diameter (MSTD) and mean testicular biopsy scoring (MTBS) maintained in a better condition. The levels of major histocompatibility complex (MHC) peptide-tetramer complex in Group C and Group D decreased significantly than Group B, while sperm density and motility were significantly higher than Group B. It was also known that the histopathological damages in Group C and Group D were less than those in Group B in the 8 weeks after operation.
UTT can cause impairment of contralateral testicular function and decrease of spermatogenic function. The mechanism may be related to ischemia-reperfusion (IR) in early stage and autoimmune response in late stage.
本研究旨在探讨单侧睾丸扭转（UTT）后对侧睾丸的功能及其可能机制。
56只大鼠随机分为四组：A组：假手术组；B组：睾丸扭转（TT）+生理盐水（NS）组；C组：睾丸扭转（TT）+环孢素组；D组：睾丸扭转（TT）+NG-单甲基-L-精氨酸（L-NMMA）组。分别在术后1周和8周切除右侧睾丸。采用生化和组织病理学评估来评价生殖细胞损伤。
与A组相比，B组丙二醛（MDA）水平及一氧化氮（NO）/一氧化氮合酶（NOS）水平显著升高。两组在组织病理学损伤以及精子密度和活力方面存在显著差异。与B组相比，D组MDA和NO/NOS水平显著降低，而平均生精小管直径（MSTD）和平均睾丸活检评分（MTBS）保持较好状态。C组和D组主要组织相容性复合体（MHC）肽四聚体复合物水平比B组显著降低，而精子密度和活力显著高于B组。还发现术后8周C组和D组的组织病理学损伤小于B组。
UTT可导致对侧睾丸功能受损及生精功能下降。其机制可能与早期的缺血再灌注（IR）及后期的自身免疫反应有关。
