Division of Integrative Biosciences & Biotechnology, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-gu, Pohang, Gyeongbuk, 37673, Republic of Korea.
Department of Mechanical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-gu, Pohang, Gyeongbuk, 37673, Republic of Korea.
Lasers Surg Med. 2020 Apr;52(4):373-382. doi: 10.1002/lsm.23138. Epub 2019 Jul 23.
Although multiphoton microscopy (MPM) can visualize both cell and extracellular matrix (ECM) structures of the skin in high-contrast without exogenous labeling, label-free MPM is usually too slow to image clinically relevant large regions. A high-speed MPM method would be beneficial for evaluating clinical skin specimens by increasing the imaging area. In this study, moxifloxacin labeling-based MPM (moxifloxacin MPM) was characterized in various human skin cancer specimens.
STUDY DESIGN/MATERIALS AND METHODS: Moxifloxacin ophthalmic solution was used for cell-labeling and MPM imaging was conducted afterwards. Moxifloxacin MPM was characterized in ex vivo normal human skin and skin cancer specimens in comparison with the label-free MPM and fluorescence confocal microscopy (FCM) using acridine orange as a labeling agent. Then, moxifloxacin MPM was applied to various ex vivo human skin cancer specimens including basal cell carcinoma (BCC), squamous cell carcinoma (SCC), dermatofibrosarcoma protuberans (DFSP). Results of moxifloxacin MPM were compared with bright-field clinical and histopathologic findings.
Moxifloxacin MPM imaged both cells and collagen in the skin, similarly to label-free MPM, but with enhanced fluorescence intensities in cells and enhanced imaging speeds. Moxifloxacin MPM imaged cells in the skin similarly to acridine orange-based FCM. Moxifloxacin MPM of various human skin cancer specimens imaged their specific cellular features. The microscopic features detected in moxifloxacin MPM were confirmed with histological images.
This observational pilot study demonstrated that moxifloxacin MPM could detect specific cellular features of various skin cancers in good correlation with histopathological images in Asian patients at the higher imaging speed than label-free MPM. Lasers Surg. Med. © 2019 Wiley Periodicals, Inc.
虽然多光子显微镜（MPM）可以在不进行外源标记的情况下对皮肤的细胞和细胞外基质（ECM）结构进行高对比度可视化，但无标记的 MPM 通常太慢而无法对临床相关的大区域进行成像。高速 MPM 方法将有利于通过增加成像区域来评估临床皮肤标本。在这项研究中，我们对各种人皮肤癌标本进行了莫昔沙星标记的 MPM（莫昔沙星 MPM）研究。
研究设计/材料和方法：使用莫昔沙星眼药水进行细胞标记，然后进行 MPM 成像。我们将莫昔沙星 MPM 与无标记的 MPM 和荧光共聚焦显微镜（FCM）进行了比较，后者使用吖啶橙作为标记剂。然后，我们将莫昔沙星 MPM 应用于各种离体人皮肤癌标本，包括基底细胞癌（BCC）、鳞状细胞癌（SCC）、隆突性皮肤纤维肉瘤（DFSP）。莫昔沙星 MPM 的结果与临床和组织病理学的亮场结果进行了比较。
莫昔沙星 MPM 可对皮肤中的细胞和胶原蛋白进行成像，与无标记的 MPM 类似，但细胞中的荧光强度增强，成像速度也提高了。莫昔沙星 MPM 可以对皮肤中的细胞进行成像，与基于吖啶橙的 FCM 类似。各种人皮肤癌标本的莫昔沙星 MPM 可以对其特定的细胞特征进行成像。在 MPM 中检测到的微观特征与组织学图像相吻合。
这项观察性初步研究表明，与无标记的 MPM 相比，莫昔沙星 MPM 可以以更高的成像速度，在亚洲患者中良好地与组织病理学图像相关联，检测出各种皮肤癌的特定细胞特征。激光外科学与医学。© 2019 威利父子公司
