Laboratory of Immunodetection and Bioanalysis, Faculty of Dentistry, University of Antioquia, Medellín, Colombia.
J Clin Periodontol. 2019 Oct;46(10):978-990. doi: 10.1111/jcpe.13173. Epub 2019 Aug 20.
This cross-sectional case-control study aimed to determine if salivary levels of lipoxin A4 (LXA4), protectin D1 (PD1), resolvin E1 (RvE1) and maresin 1 (MaR1) might constitute a reflection of periodontal health/disease status.
One hundred and two periodontitis patients and 61 healthy controls were recruited. Periodontal clinical status was determined by criteria based on full-mouth clinico-radiographical data. Salivary concentration of the analytes was calculated by enzyme-linked immunosorbent assay. The association between the biomarkers with disease status was assessed individually and adjusted for confounding using multivariate binary logistic regression models.
Significantly decreased LXA4 and increased PD1/MaR1 salivary levels were detected in periodontitis patients in comparison with healthy controls. However, no significant differences were observed for RvE1 levels between clinical groups. Clinical parameters such as probing depth, clinical attachment loss and extent were negatively correlated with LXA4, positively correlated with PD1/MaR1 and not correlated with RvE1 salivary levels. Logistic regression analyses revealed a strong/independent association of LXA4, PD1 and MaR1 salivary levels regarding disease status. Interaction effects between demographic predictor variables and salivary concentration of LXA4, PD1 and MaR1 were also identified.
The results of this study demonstrated a strong/independent association between reduced LXA4 and increased PD1/MaR1 salivary levels with periodontitis suggesting an imbalance in the specialized pro-resolving lipid mediators (SPMs) in periodontal disease.
本横断面病例对照研究旨在确定唾液中脂氧素 A4（LXA4）、保护素 D1（PD1）、解析素 E1（RvE1）和maresin 1（MaR1）的水平是否可以反映牙周健康/疾病状况。
招募了 102 名牙周炎患者和 61 名健康对照者。牙周临床状况根据全口临床放射学数据的标准确定。通过酶联免疫吸附试验计算分析物的唾液浓度。使用多元二项逻辑回归模型，单独评估生物标志物与疾病状态的相关性，并进行混杂因素调整。
与健康对照组相比，牙周炎患者的唾液 LXA4 水平显著降低，PD1/MaR1 水平显著升高。然而，两组间 RvE1 水平无显著差异。临床参数如探诊深度、临床附着丧失和程度与 LXA4 呈负相关，与 PD1/MaR1 呈正相关，与 RvE1 唾液水平无关。逻辑回归分析显示，LXA4、PD1 和 MaR1 的唾液水平与疾病状态有很强的独立关联。还发现了人口统计学预测变量与 LXA4、PD1 和 MaR1 唾液浓度之间的交互作用效应。
本研究结果表明，牙周炎患者 LXA4 降低和 PD1/MaR1 升高与牙周炎之间存在强烈的独立关联，提示牙周病中特定的促解决脂质介质（SPMs）失衡。
