Branch of Animal Husbandry, Jilin Academy of Agricultural Sciences, Gongzhuling, China.
Key Laboratory of Beef Cattle Genetics and Breeding, Ministry of Agriculture and Rural Agriculture, Gongzhuling, China.
Anim Biotechnol. 2021 Feb;32(1):21-30. doi: 10.1080/10495398.2019.1642906. Epub 2019 Jul 24.
The insulin-like growth factor binding-protein 7 (IGFBP7) has binding affinities to IGFs and is able to either positively or negatively regulate the IGFs signaling pathway. It also plays a crucial role in cell growth, differentiation and development in an IGF-independent manner. Herein, we investigated the specific regulation of the gene encoding for IGFBP7during the differentiation process of the adipocyte cells of the Yan Yellow Cattle by interfering with or by overexpressing the gene. As a result, we found that the mRNA expression levels of IGFBP7 were significantly increased during the formation of progenitor cells. In addition, the expression levels of the lipoprotein lipase (LPL) and transcription factors (PPARγ, C/EBPα) were also significantly increased. gene overexpression and RNA interfering promoted and inhibited respectively the lipid accumulation and triglyceride production in mature adipocytes, and the expression of the LPL and transcription factors (PPARγ, C/EBPα). The changes in the protein expression levels of IGFBP7 and adipogenic factors were in accord with the changes observed in the mRNA levels. In conclusion, our results indicate that IGFBP7 plays an important regulatory role in the differentiation of preadipocyte cells.
胰岛素样生长因子结合蛋白 7（IGFBP7）与 IGFs 具有结合亲和力，能够正向或负向调节 IGFs 信号通路。它还以 IGF 独立的方式在细胞生长、分化和发育中发挥关键作用。在此，我们通过干扰或过表达基因来研究 IGFBP7 编码基因在 Yan 黄牛肉用牛脂肪细胞分化过程中的特定调控。结果发现，在祖细胞形成过程中 IGFBP7 的 mRNA 表达水平显著升高。此外，脂蛋白脂肪酶 (LPL) 和转录因子 (PPARγ、C/EBPα) 的表达水平也显著升高。基因过表达和 RNA 干扰分别促进和抑制成熟脂肪细胞中的脂质积累和甘油三酯生成，以及 LPL 和转录因子 (PPARγ、C/EBPα) 的表达。IGFBP7 和脂肪生成因子的蛋白表达水平变化与 mRNA 水平观察到的变化一致。总之，我们的结果表明 IGFBP7 在脂肪前体细胞的分化中发挥重要的调节作用。
